cutting plasmids for PCR

Rico Laage rico.laage at urz.uni-heidelberg.de
Tue Apr 30 03:20:39 EST 1996


Question,
does it make sense to  cut (linearize) a plasmid prior using it as 
template in PCR ?
I was wondering if this will have an effect on deenaturation and 
annealing step.
thanx
RICO 
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