Amazing PCR results with TMAC

Steve Griffiths sgriffit at
Tue Apr 30 09:40:27 EST 1996

 Has any one noticed the extraordinary effects of adding Tetramethyl 
ammonium chloride to otherwise difficult template? From my own 
perspective I have been able to amplify a GC rich bacterial target from a 
complex tissue homogenate without any of the annoying secondary bands 
that we previously could not remove.What are your experiences?

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