FLAG-tag from Kodak

Philippe Lefebvre plefebvre at pop.univ-lille2.fr
Tue Apr 30 08:55:13 EST 1996


We have used the Flag system for two purposes: overexpression/purification of prot 
in E. coli and WB detection, gel-shift expts and others studies of the native 
prtein.  If all applications were successful in the second case, with a highly 
specific ab that gives no background, the purification business was not that 
convincing.  This was due to a very poor cleavage of the OmpA peptide, provided by 
the pF1 vector from IBI, ad thus noeasy purification of the fusion protein was 
possible using the Mab 1 antibody.  




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