What is a good Conc. of DAPI to USE for F.I.S.H.??

dasuser at PO-Box.McGill.CA dasuser at PO-Box.McGill.CA
Tue Apr 30 07:47:46 EST 1996


Hello all,

I just bought a new stock of DAPI and I have no idea what would be a
good concentration for FISH (i.e. counterstaining chromosomes).

Does anyone one have a good rule of thumb... ie. for time and conc.
etc.?

As well, what is a good solvent to disolve it in?  I think the old stock 
we had was in PBS... but I am not sure... I have heard others disolve 
the DAPI in whatever anti-fade reagent they are using.

Any and all suggestions are welcome.

Graham Dellaire
McGill University
Experimental Medicine

dellaire at odyssee.net

or popa0206 at po-box.mcgill.ca




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