help with semiQ RT-PCR
graham at biodec.wustl.edu
Tue Apr 30 14:33:13 EST 1996
> If no controls are present to ensure optimal PCR conditions or variations
> between PCR runs, then people shouldn't use the words
> quantitate/quantitative at all!
Yes, but are any controls capable ensuring lack of "variations between
PCR runs"? I believe it has been well documented that a +/- variation
can be obtained between individual block positions in the original
Personally, I find identical PCR reactions are never that. Seeing
the lack of PCR products described as any kind of "result" I find very
hard to swallow.
J. Graham PhD
Washington University of St. Louis
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