uncut vector DNA

Gabi Gerlitz gabiger at zoot.tau.ac.il
Tue Apr 30 13:42:59 EST 1996


Hello,

      you should run your DNA after you cut it withe the two enzymes in 
agarose gel. In the next well run the uncut vector. Cut out the band of 
the open vetor and elute the DNA from the agarose gel with Geneclean Kit.
Then do the ligation. 

                                         Gabi Gerlitz
                                         department of Cell Researh and
                                         Immunology,
                                         faculty of Life Sciences,
                                         Tel-Aviv University,
                                         Israel. 




More information about the Methods mailing list