cDNA quantification

James Graham graham at biodec.wustl.edu
Tue Apr 30 14:22:03 EST 1996


> Usually, we just add a small amount of 32P labeled nucleotide. Then, by
> calculating the specific activity of your cDNA, you should estimate the
> amount synthesized.

I'm finding that removing say 5ul of a 25 ul complete reaction immediately
to a second tube containing 1 ul of fresh dCTP* and incubating them in
parallel allows one to accurtely quantitate the unlabeled reaction by 
extrapolation without imparting any instability to the cDNA product.

Jim
J. Graham PhD 
Biology Department 
Washington University of St. Louis 



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