Stratagene quick change mutagenesis

Bret Sellman bsellman at microbiology.bmsb.uokhsc.edu
Tue Apr 30 17:27:09 EST 1996


We have recently started using the Stratagene Quick Change Method of
site directed mutagenesis.  The first time we did it we sequenced our
clones and they were all the parental plasmid.  Has anyone had this
problem?  If so do you have any info?  We follow the protocol exactly
and grow the plamid in a Dam methylase positive host and digest with
the prescribed amount of DPNI for the required time.
Thank you

e-mail address
bsellman at microbiology.bmsb.uokhsc.edu



More information about the Methods mailing list