Do A's really fall off over time?

John Dixon jpcd0 at
Tue Apr 30 11:31:46 EST 1996

Invitrogen state in their TA cloning protocol that one should use "fresh
(less than 1 day old) PCR products, because the single 3'A overhangs on
the PCR products will be removed over time, reducing efficiency". 

Is this for real? Will the A's be lost even if the product has been stored
at -20? 

Thanks for any tips



John Dixon                                        Lab 44 (1223) 334131
Wellcome/CRC Institute                            Fax 44 (1223) 334134
Department of Genetics
Cambridge University    
United Kingdom                           e-m: jpcd0 at

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