Do A's really fall off over time?
jpcd0 at mole.bio.cam.ac.uk
Tue Apr 30 11:31:46 EST 1996
Invitrogen state in their TA cloning protocol that one should use "fresh
(less than 1 day old) PCR products, because the single 3'A overhangs on
the PCR products will be removed over time, reducing efficiency".
Is this for real? Will the A's be lost even if the product has been stored
Thanks for any tips
John Dixon Lab 44 (1223) 334131
Wellcome/CRC Institute Fax 44 (1223) 334134
Department of Genetics
United Kingdom e-m: jpcd0 at mole.bio.cam.ac.uk
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