Methods April 1996 Archives by author

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Starting: Mon Apr 1 00:07:33 EST 1996
Ending: Tue Apr 30 23:40:11 EST 1996
Messages: 1363

Need: Protocol for Dig-labbelling PCR $user
Need: Protocol for Dig-labbelling PCR $user
Desaturation of [9,10-H3]stearyl-CoA Cary Stewart MSTP '98
buffer pH and temperature Enrique Jose Labadan Frio - SCBC - 3421101
RNA Molecular Weight Marker K J C 944
a mystry K J C 944
Problem in PCR A.Doherty
protein purification RAFAEL ALDABE
BIAcore, IAsys, Westerns AShaw5
EtBr staining acrylamide gels AShaw5
Linear acrylamide (was --Re: glycogen as a carrier) AShaw5
trizol AShaw5
tearing my hair out over IPs AShaw5
Are plasmids purified by Qiagen really that bad????? AShaw5
Sequencing Problems w/ Prism SEQUENASE terminator/abi system AShaw5
Chicken antibody purification Alex Abbas
suspension culture Mr Hassan Abdulrazzak
To Pre or not to Pre ? Mr Hassan Abdulrazzak
Mycoplasma detection kits, and mycoplasma effects Karen Achanzar
trizol Karen Achanzar
shooting yourself in the foot.... Karen Achanzar
DNA extn from high phenolic tissue? Michael Adamson
DNA in ELISA's Michael Adamson
saturated ammonium acetate Shanthi Adimoolam
saturated ammonium acetate Shanthi Adimoolam
denaturing vs. non-denaturing electrophoresis Shanthi Adimoolam
Native gel Shanthi Adimoolam
uncut vector DNA Shanthi Adimoolam
Uncut vector DNA-again Shanthi Adimoolam
IMPORTANT - Sun Microsystems and Academic Press sponsor BIOSCI!! BIOSCI Administrator
DOWNTIME - Friday 11PM to Sat 6PM PDT BIOSCI Administrator
IMPORTANT - BIOSCI Fundraising Update! BIOSCI Administrator
cfos and cun Adrian.Philbey at SMTPGWY.AGRIC.NSW.GOV.AU
GR Analysis in Potatoes - Protocol Wanted Maqbool Ahmed
NdeI: cutting problems?? anyone else?? seems silly! (fwd) Ashok Aiyar
PCR problem - disappearing bands Bill Alexander
license for TAQ Bill Alexander
license for TAQ Bill Alexander
license for TAQ Bill Alexander
license for TAQ Bill Alexander
license for TAQ Bill Alexander
license for TAQ Bill Alexander
license for TAQ Bill Alexander
Knock-out of bacterial gene Bill Alexander
Precipitation of DNA/RNA with isopropanol Bill Alexander
help with semiQ RT-PCR Bill Alexander
license for TAQ Bill Alexander
Vector as cDNA primer?? Bill Alexander
southern blot lower limit Bill Alexander
help with semiQ RT-PCR Bill Alexander
shooting yourself in the foot.... Bill Alexander
PCR-Mimics- construct kit-Internal Controls for Quantitative PCR Bill Alexander
shooting yourself in the foot.... Bill Alexander
PCR-Mimics- construct kit-Internal Controls for Quantitative PCR Bill Alexander
E. coli XL1 Blue MR follow up Mikhail Alexeyev
BL21-K12? Mikhail Alexeyev
Basic Info Mr. J.B. Mohd Ali
Molecular Probes Web Page Mario Allaire
Syntex protest Altnews
Tc inducible system Danny Altshuler
High G-C PCR...help. Eric C. Anderson
Precipitation of DNA/RNA with isopropanol Eric C. Anderson
help with northern quantitation! Eric C. Anderson
Antibodies in Australia Thomas R. Anderson
Adress Berkeley Antibody Thomas R. Anderson
Storm Imager 840 Tracy E. Anderson
murine oocyte cDNA library STUTZ Andre
Globomycin Christine Andrews
PROTOCOL REQUEST: B-gal staining of frozen thaw mounted mouse brain sections Dr. Alexander J. Annala
CTAB.... Anonymous
Are plasmids purified by Qiagen really that bad????? Lo Wing Ip Anthony
cutting plasmids for PCR Lo Wing Ip Anthony
peritoneal cell types Lo Wing Ip Anthony
Cutting Qiagen miniprebs?? Tracy Aquilla
license for TAQ Tracy Aquilla
license for TAQ Tracy Aquilla
license for TAQ Tracy Aquilla
help with semiQ RT-PCR Tracy Aquilla
shooting yourself in the foot.... Tracy Aquilla
shooting yourself in the foot.... Tracy Aquilla
EtBr staining acrylamide gels Arioch
SuperSignal - Ultra sensitivity Tehcnical Assistance
Chloroform & lambda: How and why? BOFH
unsubscribe BOYDSTON at MVAXGR.HUNTER.CUNY.EDU
unsubscribe BOYDSTON at MVAXGR.HUNTER.CUNY.EDU
Where to get DNA Sequencing Service ? Hyunsu Bae
archive search engine? Dan Baggott
Protein blocking solution for DNA hybridization Frank Bartels
Protein blocking solution for DNA hybridization Frank Bartels
Protein blocking solution for DNA hybridization Frank Bartels
Protein blocking solution for DNA hybridization Frank Bartels
"Differetial Dismay" reference L. Bastian
luminol and luminometer Robert Bateman
Knock-out of bacterial gene Jose M. Bautista
Knock-out of bacterial gene Jose M. Bautista
How many bases can be read with ALF-DNA-sequencers, who was good results with other automatic DNA-sequencers? Jose M. Bautista
How many bases can be read with ALF-DNA-sequencers, who was , good results wit Jose M. Bautista
poly-His Ab Jim Beasley
kanamycin Jan Paul Bebelman
EtBr staining acrylamide gels Robin Beech
Re- removal of unused prime Brett Beitzel
Re- amp cassette Brett Beitzel
Re- DNase cuts Brett Beitzel
Random Hexamer PCR Brett Beitzel
SuperSignal - Ultra sen Brett Beitzel
PCR Patent Debate Brett Beitzel
macrophage electroporation Michail Belyavskyi
E. coli XL1-Blue MR strain - question! Michael Benedik
Sending bacteria via mail Michael Benedik
XL1-Red in vivo mutagenesis Michael Benedik
electroporation with HEK Deslauriers Benoit
"Four-color single lane" references Mark E. Berres
a mystry Yves Bertheau
To Whom is in CNRS, France Yves Bertheau
LexA Rudi Beyaert
Knock-out of bacterial gene Tom Bickle
LacSwitch Display Systems Biotech
SuperSignal - Ultra sensitivity Mr Blue101
RNA quantification Torsten Boerchers
Glucose phosphate isomerase Dr. Eric Bouhassira
ability to pcr introns from cDNA Robot Boy
Exonuclease deletion kits...help !!! Sensational Gravity Boy
USB Address Joan Boyce
USB Address Joan Boyce
USB Address Joan Boyce
Vector Laboratories, CA - address/tel. # wanted Joan Boyce
Culture of E.Coli (SURE) / Large Plasmids / Foreign Sequences Chris Boyd
Ligation Chris Boyd
PCR enzymes Chris Boyd
Knock-out of bacterial gene Chris Boyd
Help with Plasmids and Strains Database? Scott K. Boyer
TAE vs. TBE? Sarah Tamara Braun
calyculin A Annick Breton
Cytokines Web Simon M. Brocklehurst
cloning up to 12Kb into a eukaryotic expression vector, needing RNA transcription only Andrew Brooks
Cloning up to 12kb into a eukaryotic expression vector, needing RNA transcription only. Andrew Brooks
cloning up to 12 kb into mammalian cells Andrew Brooks
cloning up to 12 kb into mammalian cells Andrew Brooks
cloning up to 12 kb into mammalian cells Andrew Brooks
Marsupial genomic library Brian Brunk
[Na] and Tm of oligos... Bill Burnett
a mystry Bill Burnett
Differential Display Tonja Burshek
RNA quantification Tonja Burshek
DNasing RNA samples Tonja Burshek
direct PCR from E. coli Geraldine Butler
SDS in western blot transfert Dr E. Buxbaum
looking for plasmids pTrcHis A,B,C (Invitrogen) CAMPEAU,ERIC,M
ignore this CKY at mail.nerc-oxford.ac.uk
testing mail CKY at mail.nerc-oxford.ac.uk
Screning of a plasmid cDNA library Manuel G. CLAROS
Another Ligation Problem Carolyn Calnan
Wanted: Peptide quantitation method From Jaume Canaves
Staged Embryos Martin Cann
Ligation Philip L. Carl
High G-C PCR...help. Steven James Carlisle
**HELP!** Plant growth medium contamination T. Hedley Carr
Heater parts for a Glass Still Eric B. Carstens
Cytochrome c extraction Ron Caspi
slide printer Ron Caspi
Plant Science Postdoctoral Position Brandt Cassidy
Automated sequencing reactions Ed Castro
WST-1,MMT assay cell viability: who knows? Ed Castro
HB-4 rotor Ed Castro
Tg mouse/rat discussion group Ed Castro
cDNA First strand synthesis Ed Castro
RNA quantification Ed Castro
Fas, Bcl-2, sgp-2 probes: where? Ed Castro
Human cDNA expression library wanted. Dr Zhonglin Chai
Expression libraries wanted (HeLa and Brain) Dr Zhonglin Chai
Expression libraries wanted Dr Zhonglin Chai
Expression libraries wanted (HeLa and Brain) Zhonglin Chai
PCR problem - disappearing bands Zhonglin Chai
cDNA cloning using expression library Zhonglin Chai
Best inducible mammalian expression vector Lisa Chakrabarti
Centrifuging MO's Alec Chambers
pZeoSV map, Help Help ASAP tamakoto Chang
which company sells plant poly(A) RNA? Jeremy Chen
ISCO and Sartorrius email addresses Andrey Chernov
RNA isolation Paul Chien
RNA Oligo supplier??? Casey Chin
Plasmids purified by Qiagen - just fine! Nikolai Chitaev
**HELP!** Binary Vector Ligations Nikolai Chitaev
Ligations with 1 cohesive & 1 blunt end...best conditions? Nikolai Chitaev
Plasmids purified by Qiagen - just fine! Nikolai Chitaev
Uncut vector DNA-again Nikolai Chitaev
calculation of % identity & % similarity in pairs of seq Jaishree Muppala Chittoor
random priming Lely Chow
hybing without shaking?? Lely Chow
Blood spot DNA Extraction John Christodoulou
AvrII and StuI cutting peculiarities? Dr. Duncan Clark
license for TAQ Dr. Duncan Clark
license for TAQ Dr. Duncan Clark
license for TAQ Dr. Duncan Clark
license for TAQ Dr. Duncan Clark
PCR enzymes Dr. Duncan Clark
archive search engine? Dr. Duncan Clark
Gene synthesis using oligos Dr. Duncan Clark
archive search engine? Dr. Duncan Clark
Gene synthesis using oligos Dr. Duncan Clark
NdeI: cutting problems?? anyone else?? seems silly! (fwd) Dr. Duncan Clark
Randomising aa's, how? Dr. Duncan Clark
license for TAQ Dr. Duncan Clark
Electroporation Dr. Duncan Clark
E. coli XL1-Blue MR strain - question! Dr. Duncan Clark
NdeI: cutting problems?? anyone else?? seems silly! (fwd) Dr. Duncan Clark
license for TAQ Dr. Duncan Clark
Long Range PCR Question Dr. Duncan Clark
High G-C PCR...help. Dr. Duncan Clark
TA cloning Dr. Duncan Clark
Linear acrylamide (was --Re: glycogen as a carrier) Dr. Duncan Clark
Linear acrylamide (was --Re: glycogen as a carrier) Dr. Duncan Clark
Single error in Primer Dr. Duncan Clark
Pouring sequencing gels the old fashioned way Dr. Duncan Clark
license for TAQ Dr. Duncan Clark
DNA precipitation by Polymine P Dr. Duncan Clark
snail genomic extraction Daun Clizbe
Alternative to autoclaving for denucleasing plasticware Wayne Coco
SOS!--PCR restriction cut Wayne Coco
Linear acrylamide (was --Re: glycogen as a carrier) Wayne Coco
(none) Coffman, Dan
"Differetial Dismay" reference Shelley Cole
RNA quantification Shelley Cole
RNA quantification Shelley Cole
Molecular Probes Web Page Computer
Miniprep SNAFU Hideously Confused
help with northern quantitation! Richard Converse
help with a c-myc tagging vector? Richard Converse
license for TAQ Michael Cooley
firefly luciferase Michael Cooley
MAP and Jun kinase assays Seth Corey
DNA sequencing service survey Seth Corey
poly-His Ab Seth Corey
Flouoragraphy for 35s Joao Vasconcelos Costa
protein blocking of DNA/RNA hybridisations Joao Vasconcelos Costa
Are plasmids purified by Qiagen really that bad????? Joao Vasconcelos Costa
Are plasmids purified by Qiagen really that bad????? Joao Vasconcelos Costa
HELP! 3-way ligation Maria C. Costanzo
Betagen address Robert Cowherd
Betagen address Robert Cowherd
Betagen address Robert Cowherd
About ligation Robert Cowherd
About ligation Robert Cowherd
About ligation Robert Cowherd
Baculovirus Crouse, Joe
SOS!--PCR restriction cut Hector Cruz-Lopez
In situ PCR - mtDNA Jim Cummins
RNA Oligo supplier??? Andrew Cunningham
conserved 5'UTR D
replicator DAB at CNSVAX.ALBANY.EDU
random primed cDNA cloning kit DAB at CNSVAX.ALBANY.EDU
PC-GENE program Lee Daesang
Are plasmids purified by Qiagen really that bad????? Richard Daines
How to crack yeast for PCR ?? Darren Schneider, MD
How to crack yeast for PCR ?? Darren Schneider, MD
How to crack yeast for PCR ?? Darren Schneider, MD
PCR problem - disappearing bands Phil Davey
primer extension David L. Haviland, Ph.D.
Help: Ribonuclease protection assays. David L. Haviland, Ph.D.
3' UTRs Intact? David L. Haviland, Ph.D.
Biotinylation of MoAbs David L. Haviland, Ph.D.
Rep (1) : Any Problems With Oligo? s?? Mr SHIRE David
Rep (3) : Any Problems With Oligo? s?? Mr SHIRE David
Rep : oligo synthesizer Mr SHIRE David
Rep : Quantitative RT-PCR Mr SHIRE David
Rep : Quantitative RT-PCR Mr SHIRE David
RAPD PCR primer for Trout? Raymond Deitz
cloning amplicons with inosine David Demezas
Help with DIG Northerns R. Rex Denton
In Vivo Excision Seema Desai
exciting bugs Julian DiAvanti
HELP! Proteins precipitates after glutathione column Michael DiDonato
Does drying of PVDF membrane help immunodetection? Catherine I. DiOrio
Correction factor for spectrophotometrical quantitation of DNA Dilip Dias
promoter deletions Dilip Dias
uncut vector removal... Dilip Dias
Electroporation Cuvettes Dilip Dias
Expand PCR and TA cloning kits? John Dixon
Do A's really fall off over time? John Dixon
RACE/Anchored PCR Colin Dolphin
exon amplification/trapping Rachelle Donn
Blocking Chloroplast RNA Edward Doran
thiamine concentration Tom Dougherty
Sending bacteria via mail Tom Dougherty
Getting cells off plates Harold Drabkin
Nonradioactive screening systems Julie Drawbridge
TE damage to DNA Julie Drawbridge
Green Fluorecent Protein gene for Reporter Tomas Drgon
Another Ligation Problem Richard J. Dudley
PROGENE/TECHNE THERMOCYCLER Richard J. Dudley
uncut vector DNA Richard J. Dudley
Dichloromethane vs. Chloroform Richard J. Dudley
ability to pcr introns from cDNA Nancy Duncan
QuickChangeTM Mutagenesis Daniel Durocher
Transfection of L and Clone 9 Cells Jason R. Dyck
Capillary transfer from polyacrylamide-gels Thomas Döring
primer extension EFDG
HB-4 rotor EFDG
HIV p24 Antigen EKERRIGNEN
PCR-Mimics- construct kit-Internal Controls for Quantitative PCR SG Edwards
PCR-Mimics- construct kit-Internal Controls for Quantitative PCR SG Edwards
2DSDSPAGE STNDS Elke
reptile antibody purification Ellen.Ariel at jcu.edu.au
Reporter Assays Elonex3 at umds.ac.uk
!!!!!! M-CSF (CSF1) PROMOTER PCR PROBLEMS !!!!!!!! MINET Emmanuel
A Budded Yeast: One or Two? Hernan Espinoza
Help - yeast plasmid rapid miniprep needed! Hernan Espinoza
"Motivation" for grad students Hernan Espinoza
About ligation Ellie Ettehadieh
promoter analysis Ellie Ettehadieh
HSV1 TK antibody PS Evans
Luciferase mRNA stabilize FANX0 at BIOMED.MED.YALE.EDU
(none) Ming Fa
Spin-label probe and distance measurement Ming Fa
BIOTIN - is it still active? Ming Fa
Peptide transfer to PDVF Ming Fa
TECAN-Genesis robot Glibert Fabrice
PROGENE/TECHNE THERMOCYCLER Glibert Fabrice
Short oligo probes for Northerns ?? Patrick HJ Falckh
Pouring sequencing gels the old fashion way Patrick HJ Falckh
Asymmetric PCR Patrick HJ Falckh
Single error in Primer Patrick HJ Falckh
FROZEN acrylamide sol'n! Patrick HJ Falckh
help with semiQ RT-PCR Patrick HJ Falckh
Northern Probe Size Patrick HJ Falckh
test-ignore Don Faller
PC-GENE program Mark Farinha
Help with Electroporation!!! Mark Farinha
peritoneal cell types Ross S Feldberg
Help - yeast plasmid rapid miniprep needed! Iain Fenton
Help wanted, antibiotic cassettes Jackie Fetherston
ANNOUNCE: High quality, low price Fmoc-amino acids from ALEXIS S. Findley
[solubilization of inclusion bodies with SDS?] Mike Finney
cloning up to 12 kb into mammalian cells Karl Fischer
protein blocking of DNA/RNA hybridisations Brian A. Flannigan
HA Tag cross reactivity L. Fliegel
RNase denaturing after protection Jiri Forejt
Commercial source for HA-probe antibodies???? Michael Forte
DHEA Receptor Howard Fox
alternative to wedge sequencing gels Amie Franklin
Thermo RT's & cDNA library (hi G+C) Amie Franklin
Miniprep SNAFU Freek
ECL on NC Freek
DNA Freek
2D gels of basic proteins Dr. Peter Friedhoff
Building a UV-transilluminator Alan Friedman
Corynebacteria media and habitat? Mark Fuller
Does drying of PVDF membrane help immunodetection? G.P.
promoter analysis ANNE GEORGE
Marathon-ready cDNAs from Clontech ANNE GEORGE
ABI 373a, sequencing, and FMC LongRanger gel STEVEN GERHARDT
Oligo price wars? Olivier Gandrillon
Single error in Primer Olivier Gandrillon
(none) Shou-Jiang Gao
Ligation Royal Botanic Gardens
Northern blots without formaldehyde Dirk Geerts
Blocking Chloroplast RNA Peter Gegenheimer
Soluble Protein with pEt Peter Gegenheimer
pouring gels the old way Szatmari George
uncut vector DNA Gabi Gerlitz
help with ligation Denise M. Gibo
Rat cancer cell lines Gil
phage display antibody Clemens Gillen
Silver Thiosulfate??????????? Dr. Randal W. Giroux
CGH Gizbab
bodipy, tamera, cy5 Michael Glass
DNA extn from high phenolic tissue Michelle Gleeson
Capillary transfer from polyacrylamide-gels Michelle Gleeson
RACE/Anchored PCR Anil K Goklaney
How many bases can be read with ALF-DNA-sequencers, who was good results with other automatic DNA-sequencers? Steven Goldberg
DNA transcription Susan Goldsworthy
His-tag protein purification Ian Goodfellow
ABI 373a, sequencing, and FMC LongRanger gel Michael C. Gorry
E. coli XL1-Blue MR strain - question! James Graham
protein blocking of DNA/RNA hybridisations James Graham
Stability Bead-Avidin-Biotin-DNA? James Graham
Vector as cDNA primer?? James Graham
Not sizing cDNA (random primed)? James Graham
Subtractive Hybridization Pitfalls? James Graham
Why low second-strand cDNA? James Graham
Thermo RT's & cDNA library (hi G+C) James Graham
Subtractive Hybridization Pitfalls? James Graham
cDNA quantification James Graham
help with semiQ RT-PCR James Graham
"Motivation" for grad students James Graham
UNBLOT Protocol GriffCouch
Finding the whole sequence . . . Todd Griffith
Marathon-Ready cDNAs from Clontech Todd Griffith
TMAC tetramethyl ammonium chloride Steve Griffiths
BIG PCR WITH TMAC Steve Griffiths
Amazing PCR results with TMAC Steve Griffiths
PCR off membranes Duke Groebe
Pouring sequencing gels the old fashion way Duke Groebe
FROZEN acrylamide sol'n! Duke Groebe
DNA Duke Groebe
slide printer Bob Gross
Tyrosine phosphatase inhibitor ? Cyril Gueydan
TNT kit with T7 RNA Pol Mark Guiltinan
EtBr staining acrylamide gels David S. Guttman
(none) Szabadkai Gyorgy
DNA/RNA extraction kits Ferland Louis H.
Are plasmids purified by Qiagen really that bad????? Ferland Louis H.
pTAQ licence Ferland Louis H.
blunt&sticky-1 Ligation? Ferland Louis H.
de-lipidization Ferland Louis H.
About ligation Ferland Louis H.
Hormones to Antibodies? Ferland Louis H.
hybing without shaking?? Ferland Louis H.
Hormones to Antibodies? Ferland Louis H.
Hormones to Antibodies? Ferland Louis H.
Sending bacteria via mail Ferland Louis H.
Syntex protest Ferland Louis H.
Screning of a plasmid cDNA library Yves HATZFELD
Help with DIG Northerns Helen Haase
Help with DIG Northerns Helen Haase
Centrifuging MO's Andrea Marie Habura
alternative to wedge sequencing gels Peggy Halleck
Soluble Protein with pEt Tina Hallis
DNA on a leash? Tina Hallis
license for TAQ Robert G. Hamilton
license for TAQ Robert G. Hamilton
license for TAQ Robert G. Hamilton
Buffer calculation Sabine Hanelt
(no subject) Eric Hanson
Brassinolide Johannes Hanson
Ni-NTA Spin Columns Neil Harris
inosine in oligonucleotides Richard Hastings
help with ligation Richard Hastings
Unusual start codons Richard Hastings
cDNA quantification Francois Hatey
in situ pcr protocol for bacteria? David Haydon
cDNA First strand synthesis Philip Heacock
(no subject) Shaun Heaphy
PCR enzymes Stacey Heiserman
PCR problem - disappearing bands KUN Hematol.Lab.
HOMEMADE MERLIN GEL PURIFICATION Paul N Hengen
Pellet paint ? Paul N Hengen
HOMEMADE MERLIN GEL PURIFICATION Paul N Hengen
HOMEMADE MERLIN GEL PURIFICATION Paul N Hengen
Molecular Probes Web site Paul N Hengen
Help wanted, antibiotic cassettes Paul N Hengen
archive search engine? Paul N Hengen
Double Helix Paul N Hengen
Knock-out of bacterial gene Paul N Hengen
Knock-out of bacterial gene Paul N Hengen
Electroporation Paul N Hengen
TA cloning Paul N Hengen
Long Range PCR Question Paul N Hengen
Pouring sequencing gels the old fashion way Paul N Hengen
POINTER TO FAQ LIST Paul N Hengen
High G-C PCR...help. Paul N Hengen
Chemiluminescent Detection Kits Paul N Hengen
Linear acrylamide (was --Re: glycogen as a carrier) Paul N Hengen
blunt&sticky-1 Ligation? Paul N Hengen
Quantitative RT-PCR Paul N Hengen
Linear acrylamide (was --Re: glycogen as a carrier) Paul N Hengen
PCR FAQ Paul N Hengen
Glycogen as a carrier Paul N Hengen
"Motivation" for grad students Paul N Hengen
blunt&sticky-1 Ligation? Mark Henteleff
blunt&sticky-1 Ligation? Mark Henteleff
How to crack yeast for PCR ?? Robert Hindges
Overexpression of 2 genes from the same vector Robert Hindges
Oligo price wars? Jody K. Hirsh
NdeI: cutting problems?? anyone else?? seems silly! (fwd) Jody K. Hirsh
ANNOUNCE: High quality, low price Fmoc-amino acids from ALEXIS Dr. Stephen Hoare
Introducing silent restriction sites Tony Hodge
TAE vs. TBE? Scott Hodgson
thiamine concentration Marcel Hoffmann
thiamine concentration Marcel Hoffmann
thiamine concentration Marcel Hoffmann
(no subject) Marcel Hoffmann
thiamine Marcel Hoffmann
Hirt extracts Marcel Hoffmann
"Motivation" & Attitude Susan Jane Hogarth
-80C Freezer Mike Holbrook
Need: Protocol for Dig-labbelling PCR Jan Hoolwerf
primer extension Ken Howe
A Budded Yeast: One or Two? Ken Howe
help with ligation Mr. Chris Hoyle
Expression of EGF receptor cytoplasmic domain zhixiang Hu
Miniprep SNAFU Wei Hu
Qiagen Maxi: Quantity Wei Hu
Hygromycin:Help!!!! Susan Hubchak
ANNOUNCE: EMBO Workshop on Molecular Biology of DNA Replication Ulrich Hubscher
Cutting Qiagen miniprebs?? M.E. Hudson
Protein co-expression Fred Hughson
EtBr staining acrylamide gels Gene Huh
VTWB PCR Machine?? Gene Huh
DHEA receptor Nick Hunt
Northerns of large RNA? Patrick Hurban
RNA Molecular Weight Marker Patrick Hurban
Molecular Probes Web site ICleme1659
Good inhibitor to RNAses? Idur
DNA/RNA extraction kits Sandrine Imbeaud
Siliconized coverslips for In Situ Hybridization Imgenfm
DNA sequencing service survey Imgenfm
cDNA cloning using expression library Takahiko Ito
promoter deletions JMARTINEZ at AZCC.ARIZONA.EDU
FROZEN acrylamide sol'n! Timothy R Jackson
fluorescent oligonucleotide source Daniel Jacobs
Library screening with oligos Asfar Jamal
PC12 cell line Jane
TAP 90 cells please? Bart Janssen
DNA precipitation by Polymine P Jean Paul Raffin, Ifremer Brest PDG-DRO-EP
For all you, electroporation fans! Jfloring at aol.com
PCR diagnose technology William Jia
SOS Call to all experinced "Sequencers" Jim
RNase protections assays. Too much salt? Jim
protocol for mRNA extraction from E. coli Jim
Soluble Protein with pEt Jim
Avidin-Biotin Reaction JohnnyHugh
fluorometer or spectrofluorometer, who sells 'em Dan Johnson
Asymmetric PCR Doug Johnson
Gene Runner Manual:HELP! Lois Johnson
Wanted: GeneRunner manual/Hastings software Lois Johnson
Help wanted, film developer & electroelutor Lois Johnson
Linear acrylamide (was --Re: glycogen as a carrier) (Dave Johnston)
pouring gels the old way Jillian Johnston
Sequencing Problems w/ Prism SEQUENASE terminator/abi system Jimbo Jones
Correction to prism sequenase inquiry Jimbo Jones
Is anyone sequencing with the Prism Sequenase DS Template Kit (w/abi system) Jimbo Jones
Nuclear Run-ON Protocol Jimbo Jones
transcriptional activity and mRNA stability Jimbo Jones
PCR problem - disappearing bands Leslie Jones
SOS Call to all experinced "Sequencers" Leslie Jones
ABI 373A Read Length Improvements Mick Jones
pAlter mutagenesis Matthew D Jonsen
Qiagen Maxi: Quantity Zophonias O. Jonsson
About ligation Zophonias O. Jonsson
Pouring sequencing gels the old fashion way Rob Jordan
Summary of "Pouring sequencing gels the old fashioned way" responses Rob Jordan
help? info re SDS effects Juliewoods
Correction factor for spectrophotometrical quantitation of DNA Vanhauwe Jurgen
Subcloning sal1 insert into pBluescript KHUANG at CHEMVX.TAMU.EDU
Single primer cloning KHUANG at CHEMVX.TAMU.EDU
pTAQ licence KHUANG at CHEMVX.TAMU.EDU
Timesaver cDNA synthesis Kit KHUANG at CHEMVX.TAMU.EDU
pTAQ licence KHUANG at CHEMVX.TAMU.EDU
TA cloning KHUANG at CHEMVX.TAMU.EDU
Sequencing phage DNA directly KHUANG at CHEMVX.TAMU.EDU
About ligation KHUANG at CHEMVX.TAMU.EDU
Glycogen as a carrier KHUANG at CHEMVX.TAMU.EDU
direct PCR from E.coli KHUANG at CHEMVX.TAMU.EDU
Do A's really fall off over time? KHUANG at CHEMVX.TAMU.EDU
Sooo has anyone found my mysterious paper yet? DANIEL Y KIM
What is Histidine tagging like? DANIEL Y KIM
Long Range PCR Question KONSTANTIN.KANYUKA at bbsrc.ac.uk
Electroporation Cuvettes Chris Kafer
GFAP immunofluoresence of C6 cell line Ari Kahn
thermo-sequenase question Sultan Kalifa
Culture of E.Coli (SURE) / Large Plasmids / Foreign Sequences Sultan Kalifa
Chloroform & lambda: How and why? Kalyanaraman
TimeSaver cDNA Synthesis Kit Stefan Kappeler
BIAcore, IAsys, Westerns Gouzel Karimova
BIAcore, IAsys, Westerns Gouzel Karimova
BIAcore, IAsys, Westerns Gouzel Karimova
Electroporation Cuvettes Dan Kearns
E. coli XL1-Blue MR strain - question! Dan Kearns
E. coli XL1 Blue MR follow up Dan Kearns
80/Base Jill Kellogg
**HELP!** Binary Vector Ligations Jill Kellogg
ANNOUNCE: High quality, low price Fmoc-amino acids Jill Kellogg
EtBr and polyacrilamide gels Ed Kennedy
Pellet paint ? Nigel Kenward
Good inhibitor to RNAses? Nigel Kenward
Software for strain collections Michael Kertesz
DNA Hyder Ali Khan
molecular biology Mr D. Kim
GenePulser Masato Kinoshita
condition of Electroporation Masato Kinoshita
Electrophoration for lymphoma cell Molt-4 Masato Kinoshita
Chemiluminescent Detection Kits Julie A. Kirihara
HB-4 rotor Leszek Andrzej Kleczkowski
PVDF: Total protein stain & scanning Dima Klenchin
Help with Electroporation!!! Dima Klenchin
Help with Electroporation!!! Dima Klenchin
Electroporation Dima Klenchin
The lower limits of hygromycin Dima Klenchin
BIOTIN - is it still active? Dima Klenchin
For all you, electroporation fans! (Was: Re: Electro-transformation of mammalian cells) Dima Klenchin
Hormones to Antibodies? Dima Klenchin
PC12 cell line Dima Klenchin
"Motivation" & Attitude Dima Klenchin
**HELP!** Binary Vector Ligations Yolande Knight
Culture of E.Coli (SURE) / Large Plasmids / Foreign Sequences Michael Koch
uncut vector removal... Michael Koch
Nonradioactive screening systems Michael Koch
Are plasmids purified by Qiagen really that bad????? Michael Koch
Pouring sequencing gels the old fashion way Michael Koch
Strange smile Roland Koelliker
125-I-Activin Katri Koli
de-lipidization Helen Korsmo
Strange smile Alexander Kraev
Terminal transferase and sequencing Alexander Kraev
cyclo sequencing Alexander Kraev
**HELP!** Binary Vector Ligations Alexander Kraev
Pouring sequencing gels the old fashion way Alexander Kraev
How many bases can be read with ALF-DNA-sequencers, who was good results with other automatic DNA-sequencers? Alexander Kraev
Sequencing phage DNA directly Alexander Kraev
Labelled oligo's for the LiCor sequencer Alexander Kraev
Pouring sequencing gels the old fashioned way Alexander Kraev
ability to pcr introns from cDNA Alexander Kraev
AvrII and StuI cutting peculiarities? Robert Kraft
ANNOUNCE: High quality, low price Fmoc-amino acids from ALEXIS Cornelius Krasel
Northwestern Joachim Kremerskothen
Tyrosine phosphatase inhibitor ? Joachim Kremerskothen
BIAcore, IAsys, Westerns Sanjay Krishnaswamy
New Company WWW sites David Kristofferson
SuperSignal - Ultra sensitivity David Kristofferson
ANNOUNCE: High quality, low price Fmoc-amino acids from ALEXIS David Kristofferson
Magnetic Particles for Separation in Biosciences and as Drug carrier David Kristofferson
blunt&sticky-1 Ligation? Georg Kroeger
blunt&sticky-1 Ligation? Georg Kroeger
Sending bacteria via mail Georg Kroeger
uncut vector DNA Georg Kroeger
Mailing bacteria Georg Kroeger
High G-C PCR...help. Jerry Kropp
Glucose phosphate isomerase Arle Kruckeberg
PVDF: Total protein stain & scanning Arle Kruckeberg
PROGENE/TECHNE THERMOCYCLER Gary Kucera
PROGENE/TECHNE THERMOCYCLER Gary Kucera
Problems in protein solubilization Huseyin Kucuktas
I'm looking for Microplate Reader's info Vladimir Yu. Kuryshev
Ligations with 1 cohesive & 1 blunt end...best conditions? Kathy L
Trizol RNA purity Kathy L
New Company WWW sites martin LEACH
oligo annealing/cloning problem martin LEACH
Peptide Nucleic Acids (PNA) martin LEACH
SOS Call to all experinced martin LEACH
New Company WWW sites martin LEACH
New Company WWW sites martin LEACH
USB Address martin LEACH
PCR off membranes martin LEACH
Getting cells off plates martin LEACH
license for TAQ martin LEACH
uncut vector removal... martin LEACH
firefly luciferase martin LEACH
promoter deletions martin LEACH
Rep (1) : Any Problems With Oligo? s?? martin LEACH
HOMEMADE MERLIN GEL PURIFICATION martin LEACH
Pellet paint ? martin LEACH
license for TAQ martin LEACH
Luciferase mRNA stabilize martin LEACH
RNA Oligo supplier??? martin LEACH
How to advertise PhD martin LEACH
Quiax-DNA-gel-extraction Rico Laage
cutting plasmids for PCR Rico Laage
DNA laddering (apoptosis) Dr. Simmen's Lab
Purifiction of total nucleic acid and protein. How?? Ratner Lab
Are plasmids purified by Qiagen really that bad????? The Wagner Lab
"Motivation" for grad students The Wagner Lab
"Motivation" for grad students The Wagner Lab
"Motivation" for grad students The Wagner Lab
Sequencing Services Besharse Lab3
testing ADN Computer Center Public Labs
testing ADN Computer Center Public Labs
Sequencing phage DNA directly John Ladasky
Debugging ARMS PCR with internal control John Ladasky
How to advertise PhD Michael Robert Ladomery
Clontech's "Capfinder PCR cDNA library construction kit": Does it work? Andrew Lange
Green Fluorecent Protein gene for Reporter Olaf Lange
Restriction enzyme digest Tim Lansing
Cutting Qiagen miniprebs?? Michael Lassner
TAE vs. TBE? Michael Lassner
TAE vs. TBE? Michael Lassner
RACE/Anchored PCR Michael Lassner
Hemin detection FALQUET Laurent
FLAG-tag from Kodak FALQUET Laurent
PCR of cDNA library Mitch Lazar
LacSwitch Andrea LeBlanc
Electroporation Cuvettes Eurie Lee
Sequence analysis programs for Linux Stephen P Lee
Radiolabelling proteins in Coli Philippe Lefebvre
Are plasmids purified by Qiagen really that bad????? Philippe Lefebvre
PEI transfection Philippe Lefebvre
(no subject) Philippe Lefebvre
FLAG-tag from Kodak Philippe Lefebvre
Native Gel Difficulty David Leggett
Purification of antibody raised against hexahistidine tagged protein Leo
Hormones to Antibodies? Michelle L. Leonce
mRNA isolation - your recommendation? Dr. Victor V. Levenson
DNA EXTRACTION. Dale Levitzke
Hormones to Antibodies? Kai Lewis
Extraction of DNA from Soil Samples Xiang Li
HMG-CoA reductase Daniel Tsi at Chung Li-hao
Terminal transferase and sequencing Dr. Jeff Lightfoot
none Jinny Lin
oligo synthesizer Linkman
Pouring sequencing gels the old fashioned way Eric W. Linton
Pouring sequencing gels the old fashioned way Eric W. Linton
poly-His Ab Jerry Litt
Conjugated luciferase Donald Lo
DNA extn from high phenolic tissue? David C. Logan
2DSDSPAGE STNDS David C. Logan
**HELP!** Plant growth medium contamination David C. Logan
Silver Thiosulfate??????????? David C. Logan
Introducing silent restriction sites Jen Logan
tearing my hair out over IPs Jen Logan
DNA EXTRACTION FROM CHLAMYDIA OR MYCOPLASMA Julie Logan
Centrifuging MO's Bunny Lover
killer gene in yeast Ray Lu
DNA isolation protocol Blanca Lupiani
TA cloning Blanca Lupiani
Gene Runner Manual:HELP! R. John Lye
Any Problems With Oligo's?? Ted M.
DNA sequencing service M.Sauerborn
Stripping W. blot membrane MALINI at JASON.UTHCT.EDU
Cell lines for transfecting IAN MCFARLANE
SV40 LT purified proteins Jorge MORENO
Siliconized coverslips for In Situ Hybridization MPBELL at MVAX.hawaii.eduaccept
Repetitive Northern Hybridization MPBELL at MVAX.hawaii.eduaccept
Klentaq DNA polymerase Mike Ma
real-time advice John MacFarlane
alternative to wedge sequencing gels S. Macfarlane
Protein blocking solution for DNA hybridization S. Macfarlane
Protein blocking solution for DNA hybridization S. Macfarlane
random priming S. Macfarlane
protein blocking of DNA/RNA hybridisations S. Macfarlane
help on Moab-enzime linkage (for ADC) Marcus Macht
Using collage to analyze blot Monica Magidin
Protein 2d structure Michael Mair
Gene synthesis using oligos Rafael Maldonado
Gene synthesis using oligos Rafael Maldonado
CTAB.... Rafael Maldonado
license for TAQ Rafael Maldonado
auditory nerve cells Titta Malinen
mitchondrial dna inheritance Roy Manns
thiamine concentration Ned Mantei
protein blocking of DNA/RNA hybridisations Ned Mantei
[Na] and Tm of oligos... Ned Mantei
Pouring sequencing gels the old fashion way Ned Mantei
Linear acrylamide (was --Re: glycogen as a carrier) Ned Mantei
hybing without shaking?? Ned Mantei
Looking for 35S-APS Jose F Gutierrez Marcos
Competent cells, Inoue method - help! Jeremy Marcus
How to crack yeast for PCR ?? Jeremy Marcus
trizol Jeremy Marcus
Help - yeast plasmid rapid miniprep needed! Jeremy Marcus
Hydrotech vacuum pump Jeremy Marcus
Sending bacteria via mail Ferenc Marincs
in situ pcr protocol for bacteria? Ferenc Marincs
Qiagen Problem (secret buffers) David Martin
dynabeads Anthony Martinez
Fixation of sequencing gels J. Pat Martinez
Green Fluorecent Protein gene for Reporter J. Pat Martinez
ANNOUNCE: High quality, low price Fmoc-amino acids from ALEXIS Jeff Mathers
plasmid cDNA library amplification Maureen_L_Ho
PCR restriction cut Bernhard Mayr
ability to pcr introns from cDNA Bernhard Mayr
How to get rid of Ethidium Bromide Helen McBride
desferrithiocin Scott Selby Med-Hem
mtDNA Scott Selby Med-Hem
PVDF: Total protein stain & scanning Scott Selby Med-Hem
SuperSignal - Ultra sensitivity Scott Selby Med-Hem
Double Helix Gerardo Medrano
Reply to RNA ISOLATION FROM WHOLE ORGANISM Gerardo Medrano
Trizol RNA Purity Gerardo Medrano
Post-doctoral position Rajesh K. Mehra
Band-shift assay with U937 Ralph Melcher
Plasmid Reference Center Castor Menendez
SOS Call to all experinced "Sequencers" Pierre-Alain Menoud
Dog pancreas microsomes, commercial source Kojo Mensa-Wilmot
how to get rid of ethidium bromide??? P. Mertens
phage display library P. Mertens
Quantitative RT-PCR on blood P. Mertens
Exonuclease deletion kits...help !!! David Micklem
About ligation David Micklem
About ligation David Micklem
PFGE references? Microplate at eworld.com
Nested deletion: kits? - suggestions? Mark R. Miglarese
transcriptional activity and mRNA stability Mark R. Miglarese
uncut vector DNA Mark R. Miglarese
mitochondrial dna Mr Vid Mohan-Ram
mg+2/ca+2 atpase Mr Vid Mohan-Ram
real-time advice Mr Vid Mohan-Ram
shooting yourself in the foot.... Mr Vid Mohan-Ram
mitchondrial dna inheritance Mr Vid Mohan-Ram
2D Protein Gels Mohanish at pharmdec.wustl.edu
Fluorography for 35s labelled proteins Mr Nicholas Moody
Flouoragraphy for 35s Nick Moody
Help with DIG Northerns Gary Moore
Help with DIG Northerns Gary Moore
Help with DIG Northerns Gary Moore
Help with DIG Northerns Gary Moore
Help with DIG Northerns Gary Moore
Help with DIG Northerns Gary Moore
Help with DIG Northerns Gary Moore
Help with DIG Northerns Gary Moore
Help with DIG Northerns Gary Moore
Help with DIG Northerns Gary Moore
Help with DIG Northerns Gary Moore
Help with DIG Northerns Gary Moore
Help with DIG Northerns Gary Moore
Help with DIG Northerns Gary Moore
Help with DIG Northerns Gary Moore
Help with DIG Northerns Gary Moore
Help with DIG Northerns Gary Moore
Help with DIG Northerns Gary Moore
Help with DIG Northerns Gary Moore
Help with DIG Northerns Gary Moore
Rasmol crystal structures?? Mr. G. Morley
PDB uk mirror sites Mr. G. Morley
Pouring sequencing gels the old fashion way Scott Moye-Rowley
Quantitative RT-PCR Paul Mozdziak
PCR-Mimics- construct kit-Internal Controls for Quantitative PCR Paul Mozdziak
Magnetic Particles for Separation in Biosciences and as Drug carrier Mueller-Schulte
NEW technologies for affinity chromatography, immunoadsorbents, biocompatible polymers! Mueller-Schulte
Biotinylation of MoAbs Mick N. Mulders
ABI 377 question Kevin Munnelly
Linker add., PCR amplif., cloning, seq'ng -> degraded R.E. sites? Pete Muriana
Linker add., PCR amplif., cloning, seq'ng -> degraded R.E. sites? Pete Muriana
Subtractive Hybridization Pitfalls? Pete Muriana
Subtractive Hybridization Pitfalls? Pete Muriana
GST-Fusion protein proteolysis Brian J Murphy
ELISA plate washers Brian J Murphy
plasmid cDNA library amplification TJ Murphy
Oligo price wars? TJ Murphy
TNT kit with T7 RNA Pol Bernard Murray
license for TAQ Bernard Murray
PCR problem - disappearing bands Bernard Murray
Help wanted, antibiotic cassettes Bernard Murray
weird, strange and inexplicable membrane proteins recovery. Bernard Murray
trizol Bernard Murray
Sequence analysis programs for Linux Bernard Murray
SuperSignal - Ultra sensitivity Bernard Murray
license for TAQ Bernard Murray
trizol Bernard Murray
license for TAQ Bernard Murray
killer gene in yeast Bernard Murray
pZeoSV map, Help Help ASAP Bernard Murray
help with semiQ RT-PCR Bernard Murray
Getting rid of Proteinase K????? Bernard Murray
Labelled oligo's for the LiCor sequencer Tony Murray
lipoproteins/colloid gold labeling N.P.Dantuma at biol.ruu.nl
Suramin (Michael) Tokio Nakane
Labelled oligo's for the LiCor sequencer Your Name
(none) Pratima Nangia-Makker
Pouring sequencing gels the old fashion way Chia Jin Ngee
High G-C PCR...help. Rae Nishi
PCR Mutagenesis Jacques OBERTO
Baculovirus Yasushi OKADA
cDNA quantification Myriam Onno
Specific transcription inhibitor Stephane Ory
Phospholipase B substrate (s) ? Stephane Ory
test message.. Osama
PCR Osama
Gene Expression and mRNA stability Osama
DNA extraction from formalin-fixed brain tissue P.Orange
Fixation of sequencing gels GILLIAN NI BHARLO BOTANY PG
motivation of grad students GILLIAN NI BHARLO BOTANY PG
NdeI: cutting problems?? anyone else?? seems silly! (fwd) PRETORIUS
Linear acrylamide (was --Re: glycogen as a carrier) Suzy Paalman
HB-4 rotor Mick Partis
Commercial source for HA-probe antibodies???? Pramathesh S. Patel
DEAE-transfection of EL-4 cells Paul D. Boyer, Ph.D.
Pouring sequencing gels the old fashion way Karen Peck-Miller
PCR from fixed tissue Catherine Peichel
EtBr staining acrylamide gels Jeffrey Pepe
SOS Call to all experinced Daniel Pereira
Screening for DNA binding protein Daniel Pereira
Spin-label probe and distance measurement Eduardo Perozo
EtBr staining acrylamide gels Pete
DNasing RNA samples Pete
Help with Electroporation!!! Peter
cDNA suddnly smaller??!! Peter
Help with Electroporation!!! Peter
Drying sequencing gels Petur Henry Petersen
Need: Protocol for Dig-labbelling PCR Petur Henry Petersen
help with semiQ RT-PCR Robert K Furse PhD
help with semiQ RT-PCR Robert K Furse PhD
Incorporation of BrdU Tom Phelan
HB-4 rotor Tom Phelan
HB-4 rotor Tom Phelan
DNA extraction from formalin-fixed brain tissue SM Pickering-Brown
CTAB method of DNA purification Eva Pietrzak
hybridization question Eva Pietrzak
need info on superantigens Eva Pietrzak
Northerns of large RNA? Kay A. Pleyte
Timesaver cDNA synthesis Kit Kay A. Pleyte
Betagen address Eyck Poetter
Adress Berkeley Antibody Eyck Poetter
Help Address Sarasep Inc Eyck Poetter
Help: Endocrinology Eyck Poetter
Correction factor for spectrophotometrical quantitation of DNA Cecilia Díaz Pohl
Help with Clonetech Leandro Peral Pontigo
lowest hygromycin concentration Andrei Popov
How to crack yeast for PCR ?? Mark Prescott
abi-autoseq problems Kiley R. Prilliman
GenePulser Kiley R. Prilliman
How to crack yeast for PCR ?? Kiley R. Prilliman
help with ligation Kiley R. Prilliman
help with ligation Kiley R. Prilliman
NdeI: cutting problems?? anyone else?? seems silly! (fwd) Kiley R. Prilliman
Electroporation Kiley R. Prilliman
Are plasmids purified by Qiagen really that bad????? Kiley R. Prilliman
the best thermal cyclers Kiley R. Prilliman
inosine in oligonucleotides Robert H. Proctor
GST and Glutathione Sepharose 4B Tanel Pungas
Cutting Qiagen miniprebs?? QIAGEN
reproduciblity silver staining in native gradient gels P.H.A. Quax
Pouring sequencing gels the old fashion way Absolut Queer
HOMEMADE MERLIN GEL PURIFICATION Chanda Eva R
ABI 373a, sequencing, and FMC LongRanger gel R.Tate
saturated ammonium acetate HIRANYA ROYCHOWDHURY
saturated ammonium acetate HIRANYA ROYCHOWDHURY
cDNA First strand synthesis HIRANYA ROYCHOWDHURY
Fluorography for 35s labelled proteins HIRANYA ROYCHOWDHURY
Restriction enzyme digest HIRANYA ROYCHOWDHURY
pKa of SDS HIRANYA ROYCHOWDHURY
scFv cloning and expression - problem with pseudogenes RVDESHP0 at pwinet.upj.com
Help with DNA extractions Jimmy Rail
Protein co-expression Raj
BL21-K12? Keith Rand
Help with high mol wt protein stds Vidhya Rangaswamy
pTAQ licence Colin Rasmussen
Pouring sequencing gels the old fashion way Colin Rasmussen
pTAQ licence Colin Rasmussen
hybing without shaking?? Colin Rasmussen
Needed: Protein that ppts at less than 25% NH4SO4 Randy Rasmussen
High G-C PCR...help. Randy Rasmussen
license for TAQ Achim Recktenwald
Native gel Achim Recktenwald
Pouring sequencing gels the old fashion way Achim Recktenwald
In Situ HELP Chris Regan
Library screening with oligos Tanya Reichert
Electroporation Christian Reiser
Removal of unused primers from PCR rxn? Mary P. Remington
PCR optimization kits? Mary P. Remington
PVDF: Total protein stain & scanning Phillip J Robinson
FROZEN acrylamide sol'n! Phillip J Robinson
[solubilization of inclusion bodies with SDS?] Mike Rogers
Help: 2 Plasmid Electroporation /Infection Rolf
killer gene in yeast Rolf
Qiagen Maxi: Quantity Gerd Romanowski
Qiagen Maxi: Quantity Gerd Romanowski
5'RACE kit Gabriel Romero
test mail please ignore Ruud Ross
this is a testmail please ignore Ruud Ross
testing mail to methods@daresbury.ac.uk,please ignore Ruud Ross
test mail please ignore Ruud Ross
How many bases can be read with ALF-DNA-sequencers, who was good results with other automatic DNA-sequencers? Ruud Ross
fluorometer or spectrofluorometer, who sells 'em Robert Roxby
help with semiQ RT-PCR Janet E. Rubin
help with semiQ RT-PCR Janet E. Rubin
test, please ignore Markus Ruf
RNA Molecular Weight Marker Markus Ruf
Restriction digest software Peter Russell
uncut vector DNA George Rutherford
125-I-Activin S.
Getting cells off plates S.K.Green
Need: Protocol for Dig-labbelling PCR SCComella
Company that makes long polyd[T]? Robert SCHROCK
Screning of a plasmid cDNA library M.W. SMITH
killer gene in yeast THARUN SUNDARESAN
Weird, bizzare, inexplicable sequencing problem Sarven Sabunciyan
RNA isolation Sarven Sabunciyan
RACE Klaus Salger
killer gene in yeast Klaus Salger
FW: How to crack yeast for PCR ?? Jasper Saris
protein blocking of DNA/RNA hybridisations Keith Savin
Gel Imaging Systems Greg Schaefer
!!!!!! M-CSF (CSF1) PROMOTER PCR PROBLEMS !!!!!!!! Heinz-Juergen Schaefers
Electro-transformation of mammalian cells Heinz-Juergen Schaefers
Long Range PCR Question Martin Schenker
Sequencing phage DNA directly Martin Schenker
How to crack yeast for PCR ?? Darren Schneider
How to crack yeast for PCR ?? Darren Schneider
How to crack yeast for PCR ?? Darren Schneider
How to crack yeast for PCR ?? Darren Schneider
How to crack yeast for PCR ?? Darren Schneider
3' UTRs Intact? Robert A. Schoenfeld
(none) Steve Schramm
Comparison of thermocyclers Jan Schroeder
PROGENE/TECHNE THERMOCYCLER Jan Schröder
PROGENE/TECHNE THERMOCYCLERS Jan Schröder
PROGENE/TECHNE THERMOCYCLER Jan Schröder
PROGENE/TECHNE THERMOCYCLER Jan Schröder
PROGENE/TECHNE THERMOCYCLER Jan Schröder
** PROGENE/TECHNE THERMOCYCLERS ** Jan Schröder
[solubilization of inclusion bodies with SDS?] Axel Schumacher
trizol GE Schwab
license for TAQ Geoff Scopes
MD: Molecular Biology Position Available John L. Seed
Stratagene quick change mutagenesis Bret Sellman
Fermenter Andrei Serban
Help: method of inactivation Anatoly Sharipo
HELP - Frrze-drying of Lymphocytes. G K Sharma
scFv cloning and expression - problem with pseudogenes Regina Shaw
Make-your-own Linear acrylamide (was --Re: glycogen as a carrier) SIZE?? Glen Shearer
Culture of E.Coli (SURE) / Large Plasmids / Foreign Sequences Joan Shields
cyclo sequencing Joan Shields
FROZEN acrylamide sol'n! Joan Shields
mtDNA primers - Call for Marathon!!! Benny Shomer
mtDNA Benny Shomer
In situ PCR - mtDNA Benny Shomer
license for TAQ Song-Kun Shyue
Help! Image process software need! Song-Kun Shyue
DNA purification from gram-positives question Igor Sidorenkov
Do you know? Kurt Sims
Corynebacterium media/habitat? R.A. Skuce
Corynebacteria media and habitat? R.A. Skuce
Corynebacterium media/habitat? R.A. Skuce
Sequencing quotes needed H.J. Sluiman
Speed cruise web bioresources Cynthia S. Smagula
in vitro packaging of cosmid Dr. Koen De Smet
Sending bacteria via mail Koen De Smet
hybing without shaking?? Pam Snyder
PCR Hell Pam Snyder
trizol Pamela J Snyderer
PCR hood Pamela J Snyderer
DNA extraction from formalin-fixed brain tissue Pamela J Snyderer
High G-C PCR...help. Pamela J Snyderer
The lower limits of hygromycin Haunani A. Soares
Celery cDNA wanted Daniel Solaiman
Precipitation of DNA/RNA with isopropanol Stein Tore Solem
[solubilization of inclusion bodies with SDS?] Tan Nguan Soon
Peptide Nucleic Acids (PNA) Keld Sorensen
Wanted: Peptide quantitation method Keld Sorensen
Avidin-Biotin Reaction Keld Sorensen
tearing my hair out over IPs Keld Sorensen
Chicken antibody purification Keld Sorensen
Does drying of PVDF membrane help immunodetection? Keld Sorensen
help on Moab-enzime linkage (for ADC) Keld Sorensen
Help wanted, film developer & electroelutor Keld Sorensen
Betagen address Keld Sorensen
Chemiluminescent Detection Kits Keld Sorensen
human c-rel & p65 Poul Sorensen
Difficult transfert in western blot Giorgio Spagnol
Cloning problems with nls-lacZ Martin Spiegel
CTAB.... Silvia Spitzer
Electroporation Cuvettes Jon Stewart
FW: How to crack yeast for PCR ?? Stewart, Robert
Chicken antibody purification Wendy Stewart
saturated ammonium acetate Claudia Strehl
saturated ammonium acetate Claudia Strehl
saturated ammonium acetate Claudia Strehl
Role of serine in smooth muscle contraction Suleyman
Linker add., PCR amplif., cloning, seq'ng -> degraded R.E. sites? Sailesh Surapureddi
saturated ammonium acetate Sailesh Surapureddi
saturated ammonium acetate Sailesh Surapureddi
saturated ammonium acetate Sailesh Surapureddi
searching for scientist's e.mail address Sailesh Surapureddi
Are plasmids purified by Qiagen really that bad????? Sailesh Surapureddi
Pouring sequencing gels the old fashion way Sailesh Surapureddi
replicator Sailesh Surapureddi
Are plasmids purified by Qiagen really that bad????? Sailesh Surapureddi
Help with high mol wt protein stds Sailesh Surapureddi
Uncut vector DNA-again Sailesh Surapureddi
Short oligo probes for Northerns ?? Claudia Sutton
HELP! 3-way ligation Sean Sweeney
UNBLOT Protocol KELLY THOME
Help: 2 Plasmid Electroporation /Infection TJ
-80C Freezer Brian W. Tague
unsubscribe Noriaki Tamura
Are plasmids purified by Qiagen really that bad????? Seng-Lai Tan
Are plasmids purified by Qiagen really that bad????? Seng-Lai Tan
Plasmids purified by Qiagen - just fine! Seng-Lai Tan
Sooo has anyone found my mysterious paper yet? Song Tan
help with ligation Jil Tardiff
HB-4 rotor Ron Tate
HB-4 rotor Ron Tate
HB-4 rotor Ron Tate
Native gel Ron Tate
thiamine concentration Richard B. Taylor
TE degradation of DNA Richard B. Taylor
TE damage to DNA Richard B. Taylor
DNA sequencing service survey Dennis Templeton
NIH grant templates for Mac Word 5 and Excel available Dennis Templeton
Searching for a CAT antibody ! Th.Neumann
FROZEN acrylamide sol'n! Tom Thatcher
[Na] and Tm of oligos... John R. Thompson
southern blot lower limit Michael W. Thompson
help with ligation Anthony Tomlinson
NdeI: cutting problems?? anyone else?? seems silly! (fwd) Anthony Tomlinson
PCR enzymes Tori
Green Fluorecent Protein gene for Reporter Toshio
differential display PCR Renee Trudeau
Purifiction of total nucleic acid and protein. How?? James Tsuruta
Soluble Protein with pEt-Sorbitol/Betaine Bradley Turner
WST-1,MMT assay cell viability: who knows? Bradley Turner
Marathon/RACE Experiences? Bradley Turner
Methyl Mercury Hydroxide--Supplier Bradley Turner
Linear acrylamide (was --Re: glycogen as a carrier) SIZE?? Bradley Turner
Vector Laboratories, CA - address/tel. # wanted Simon Twigger
Vector Laboratories, CA - address/tel. # wanted Simon Twigger
LCR (Ligase Chain Reaction) kit Paul Ulanch
PCR Mutagenesis Unknown
ribosomal RNA in DD Markku Vaarala
help with ligation Edgar Valencia
peritoneal cell types Edgar Valencia
REQ: rat interleukin-6 antibody Luc Vallières
Expand PCR and TA cloning kits? Margon Vandongen
Pellet paint ? Margon Vandongen
Precipitation of DNA/RNA with isopropanol Margon Vandongen
help on Moab-enzime linkage (for ADC) Rodrigo Cassio Sala Veneziani
Sf9 cDNA library Mark Vickers
Sf9 cDNA library Mark Vickers
the best thermal cyclers Marc Visconti
LOOKING FOR GLUTAMATE RECEPTOR PROTEIN expressing gene... Matthew J Vona
Need Pure Histone-core Matthew J Vona
Culture of E.Coli (SURE) / better areation / Tunair Flaxk WSchick at aol.com
Peptide Nucleic Acids (PNA) WSchick at aol.com
Heater parts for a Glass Still WSchick at aol.com
license for TAQ WSchick at aol.com
fluorometer or spectrofluorometer, who sells 'em Turner Designs WSchick at aol.com
How many bases can be read with ALF-DNA-sequencers, who was good results wit WSchick at aol.com
EtBr staining acrylamide gels/with sample WSchick at aol.com
Rep : oligo synthesizer WSchick at aol.com
Hydrotech vacuum pump WSchick at aol.com
Betagen address WSchick at aol.com
DNA EXTRACTION. WSchick at aol.com
PROGENE/TECHNE THERMOCYCLER WSchick at aol.com
license for TAQ WSchick at aol.com
shooting yourself in the foot.... WSchick at aol.com
Mycoplasma detection kits, and mycoplasma effects Paul Wakenight
Qiagen Problem (secret buffers) Paul Wakenight
Trizol RNA purity Edward Wang
PCR FAQ Edward Wang
a mystry Edward Wang
N-acetyl-galactosaminolactone source? Peter Wang
cre-lox vectors cause recombination? Shiao Y. Wang
abi-autoseq problems Lawrence Washington
Labelled oligo's for the LiCor sequencer Lawrence Washington
Extra Long PCR reamplification Russell Floyd Watkins
Gene synthesis using oligos mike Watson
*** CAN ANYONE EXPLAIN THIS????*** Dr Mark Watson
***ADVICE ON VIRAL RNA PREPARATION PLEASE!***** Dr Mark Watson
HELP - RT-PCR David Webb
proteinase pretreatment??? Chi-Kuang Wen
proteinase pre-treatment Chi-Kuang Wen
protocol for mRNA extraction from E. coli Chi-Kuang Wen
Subtractive Hybridization Pitfalls? Chi-Kuang Wen
Wanted: Peptide quantitation method Geoffrey D. Wheelock
Centrifuging MO's Michal White
BIOTIN - is it still active? Malgorzata Wilczynska
Peptide transfer to PDVF Malgorzata Wilczynska
promoter active in both gram+/- bacteria Moens William
protein purification Randall Willis
FROZEN acrylamide sol'n! Randall Willis
Sending bacteria via mail Randall Willis
"Motivation" for grad students Randall Willis
Second Electronic Glycoscience Conference Iain Wilson
Recombinant phage antibody system Troels Wind
Randomising aa's, how? Troels Wind
phage display antibody Troels Wind
phage display library Troels Wind
CTAB.... David Winterbourne
CTAB.... David Winterbourne
pcr licenses John Wise
Help - yeast plasmid rapid miniprep needed! Stephan Witte
tearing my hair out over IPs Arthur Wohlwill
PCR enzymes Jason Wooden
MAP and Jun kinase assays Jim Woodgett
Recombinant phage antibody system Chao-Liang Wu
DH5alpha E coli cells Elli Wurtzel
pComb3H/Fab library QUESTION? X
5'RACE kit Mayumi Yagi
Problems with ABI sequencer. Help !!!!! Keun-Hang Yang
help with ligation Victor Yankiwski
MW of mouse IgG and protein A in IP Tang Hsin Yao
test, please ignore Jun Yasuda
Betagen address Jun Yasuda
Hirt extracts Ian A. York
MAP and Jun kinase assays David Tak Yan Yu
cfos and cun David Tak Yan Yu
Delta Fingerprinting David Tak Yan Yu
PCR to detect mycoplasma David Tak Yan Yu
electroporator Pak-Lam Yu
Retic. lysate for degradation? Matthias Zeiner
ANNOUNCEMENT (ETRO Working Party on genetics and vascular diseases) Francesco Zito
mystery band pCDNA3/EcoRI/-PO4 Frances Hannan Zoology
Chloroform & lambda: How and why? acatalan at EXTRO.UCC.SU.OZ.AU
Dichloromethane vs. Chloroform acatalan at EXTRO.UCC.SU.OZ.AU
Suramin source arturo.galvani at itner.pharmacia.se
Growing Containers!! awb at aber.ac.uk
Growing Containers!! awb at aber.ac.uk
molecule sebastien bergeron
DNA Extraction from frozen mammalian tissue bjh8 at Lehigh.EDU
Getting rid of Proteinase K????? bjh8 at Lehigh.EDU
Automated RNA isolation needed books at mcvax2.d48.lilly.com
cDNA library..help john brennand
cDNA library..help john brennand
plasmid cDNA library amplification john brennand
USB Address john brennand
molecular biology john brennand
Ligation john brennand
Correction factor for spectrophotometrical quantitation of DNA john brennand
Protein co-expression john brennand
Test - please ignore john brennand
GenePulser john brennand
GenePulser john brennand
archive search engine? john brennand
archive search engine? john brennand
Electroporation Cuvettes john brennand
Help: 2 Plasmid Electroporation /Infection john brennand
help with ligation john brennand
help with ligation john brennand
Radiolabelling proteins in Coli john brennand
Miniprep SNAFU john brennand
Radiolabelling proteins in Coli john brennand
cDNA First strand synthesis john brennand
PCR patent debate - part 73 john brennand
pTAQ licence john brennand
pTAQ licence john brennand
Qiagen Maxi: Quantity john brennand
Precipitation of DNA/RNA with isopropanol john brennand
OD ratios of 3 to 4!? john brennand
SuperSignal - Ultra sensitivity john brennand
Qiagen Maxi: Quantity john brennand
Sequencing phage DNA directly john brennand
DH5alpha E coli cells john brennand
Suramin (Michael) john brennand
Suramin (Michael) john brennand
PCR of cDNA library john brennand
Dog pancreas microsomes, commercial source john brennand
Vector as cDNA primer?? john brennand
5'RACE kit john brennand
Another Ligation Problem john brennand
Another Ligation Problem john brennand
Not sizing cDNA (random primed)? john brennand
Qiagen Problem (secret buffers) john brennand
Growing Containers!! john brennand
de-lipidization brett
Sending bacteria via mail brett
"Motivation" for grad students brett
"Motivation" & Attitude brett
DNase cuts luis brieba
T7 RNA Pol in vivo assay luis brieba
Review Antibody Production lena brydon
To Whom is in CNRS, France byeongha at PLAZA.SNU.AC.KR
Quinol chan at bioch.ox.ac.uk
TE damage to DNA corboy at utdallas.edu
uncut vector DNA corboy at utdallas.edu
BL21-K12? crbaker
brain slices for Ab stain - company? hong dang
What is a good Conc. of DAPI to USE for F.I.S.H.?? dasuser at PO-Box.McGill.CA
archive search engine? davesmith
"Differetial Dismay" reference ege
southern blot lower limit elida at parasit.lan.mcgill.ca
ss vs ds DNA bjorn eriksson
sds effect on tissue storage julie feinstein
can normal peripheral B cells be transfected ? fouret
Address for Biocarb company frosc000 at mzdmza.zdv.uni-mainz.de
peptide gel gc
help with cDNA. aparjita ghosh
phage display library sujoy ghosh
Restriction enzyme digest sujoy ghosh
GENE REARRANGEMENT PROBES? kevin gilbride
Suramin (Michael) glycotech
Hydrotech vacuum pump harvey
FLAG-tag from Kodak harvey
how to get rid of ethidium bromide??? john haynie
Where to get DNA Sequencing Service ? helix.nih.gov
Klentaq DNA polymerase helix.nih.gov
Sequencing quotes needed iddavis at vms.cis.pitt.edu
killer gene in yeast Administrador de iihema.fmed.uba.ar
Methyl Mercury Hydroxide Administrador de iihema.fmed.uba.ar
Ceramide measuring Administrador de iihema.fmed.uba.ar
Recombinant phage antibody system ijiwaru at wheel.dcn.davis.ca.us
tearing my hair out over IPs ijiwaru at wheel.dcn.davis.ca.us
ANNOUNCEMENT: Understanding Protein Structure Determination Lennart Nilsson (Karolinska institutet)
Vector Laboratories, CA - address/tel. # wanted jamesm at holyrood.ed.ac.uk
SOS Call to all experinced janke at gen.lu.se
Help Needed with Library Screening jchristn at umich.edu
microwaves jean
Reporter Assays joestudent at mit.edu
Hygromycin:Help!!!! kim myung jong
Avidin-Biotin Reaction karimova
abi-autoseq problems aaron jacob kelly
test please ignore alan kong
RNA-binding proteins krafft at uni-muenster.de
RNA-binding protein krafft at uni-muenster.de
cloning of YAC DNA larsen at CALSHP.CALS.WISC.EDU
trizol lcwilliams at medusa.unm.edu
Agbiotech Conference Saskatoon (fwd) lees at SASK.USASK.CA
PVDF: Total protein stain & scanning logan
email addr. at Univ. of Bielefeld (Germany) lonnie
Lactococcus peptidoglycan prep. lothar at lmb1.rug.ac.be
Tetanos toxin frag. C lothar at lmb1.rug.ac.be
Avidin-Biotin Reaction madbrow
weird, strange and inexplicable membrane proteins recovery. mazella at naxos.unice.fr
Problem in PCR mdbcs002 at CMU1.CHIANGMAI.AC.TH
"Motivation" for grad students me.
uncatalyzed phospholipase-D reaction phillip messersmith
test-please ignore michael_weiner at glaxo.com
how to get rid of ethidium bromide??? mikem at s-and-s.com
PCR of cDNA library mikem at s-and-s.com
Searching for a CAT antibody ! milstone
Green Fluorecent Protein gene for Reporter milstone
Peptide transfer to PDVF ming at msvax.mssm.edu
Weird, bizzare, inexplicable sequencing problem mkennedy at chmeds.ac.nz
test mook
anti darc antibody anti-Fy6 chris moore
killer gene in yeast mpk6
SuperSignal - Ultra sensitivity your name
Native Gel Difficulty nc26 at wwa.com
Nonreducing Gel nc26 at wwa.com
PCR problem - disappearing bands ndxdbmay at rrzn-user.uni-hannover.de
XL1-Red in vivo mutagenesis neuro
lipid mediated transfection of fish cells kee ng
saturated ammonium acetate nsaunders at molbiol.ox.ac.uk
Quantification beta-glucan content Gabriela Martinez Trevi o
Obtention ribosomal subunits Gabriela Martinez Trevi o
obtention ribosomal subunits Gabriela Martinez Trevi o
Obtention ribosomal subunits Gabriela Martinez Trevi o
Obtention ribosomal subunits Gabriela Martinez Trevi o
Looking for TM Analytic okada at ouvaxa.cats.ohiou.edu
NdeI: cutting problems?? anyone else?? seems silly! (fwd) gerry oneill
NdeI cutting BIG HUMBLE APOLIGY gerry oneill
snail genomic extraction pdxrmb at vaxa.nott.ac.uk
S1 Nuclease chip peele
Klentaq DNA polymerase pgt
Pouring sequencing gels the old fashion way pubh021
Nested deletion: kits? - suggestions? radomski
uncut vector removal... rhubner at molbiol.ox.ac.uk
Corynebacteria media and habitat? richard
faint PCR bands rlee
Miniprep SNAFU jane sandall
tearing my hair out over IPs simon
Biotinylation of MoAbs simon
searching for scientist's e.mail address sl3wx at cc.usu.edu
partial cleavage by Thrombin sl3wx at cc.usu.edu
EtBr staining acrylamide gels holin sue
How to crack yeast for PCR ?? Michael Liss t4310
Help with Electroporation!!! tera
Help with Electroporation!!! tera
test ignore tflanigan at molbiol.ox.ac.uk
OD ratios of 3 to 4!? tflanigan at molbiol.ox.ac.uk
antibodies to K channel subunits? tflanigan at molbiol.ox.ac.uk
proteinase pretreatment??? mark throsby
Sequencing Services natalie unruh
Rep : oligo synthesizer natalie unruh
Rep : oligo synthesizer natalie unruh
PCR hood upyers
Anti-Human Cytokine X-Reacts with Simian Cytokines? upyers
PCR off membranes varyc.mmcri at mmc.org
cosmid amplification vdilbeck at dbm.ulb.ac.be
Western blots olle vidal
RACE vilimf01 at mcrcr6.med.nyu.edu
RACE/Anchored PCR vilimf01 at mcrcr6.med.nyu.edu
cloning amplicons with inosine vilimf01 at mcrcr6.med.nyu.edu
FROZEN acrylamide sol'n! vilimf01 at mcrcr6.med.nyu.edu
About ligation vilimf01 at mcrcr6.med.nyu.edu
pK18 plasmid info vilimf01 at mcrcr6.med.nyu.edu
Sequence Analysis Software vipond at BSA.BRIS.AC.UK
Northern blots without formaldehyde ruth e.w. walker
Help about blood storage please! ruth e.w. walker
Protein blocking solution for DNA hybridization ruth e.w. walker
Northerns of large RNA? ruth e.w. walker
trizol ruth e.w. walker
thiamine concentration wally at bio.tamu.edu
cDNA First strand synthesis walter
Gibco Stbl2 cells: deletion curse! walter
v-abl and phosphatase assay annette walter
EtBr staining acrylamide gels williams at bscr.uga.edu
In Vivo Excision Ron Goto x2832
random priming Ron Goto x2832
random primed directional cDNA library xxxxxxx
protocol for mRNA extraction from E. coli xiaoming yi
alternative to wedge sequencing gels xiaoming yi
alternative to wedge sequencing gels xiaoming yi
RNA isolation from whole organism rather than tissue or cells john yon

Last message date: Tue Apr 30 23:40:11 EST 1996
Archived on: Sun Mar 6 21:02:25 EST 2005

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