Methods April 1996 Archives by thread
Starting: Mon Apr 1 00:07:33 EST 1996
Ending: Tue Apr 30 23:40:11 EST 1996
Messages: 1363
- PFGE references?
Microplate at eworld.com
- Recombinant phage antibody system
Chao-Liang Wu
- Cutting Qiagen miniprebs??
Michael Lassner
- Introducing silent restriction sites
Tony Hodge
- cDNA library..help
john brennand
- cDNA library..help
john brennand
- plasmid cDNA library amplification
john brennand
- Getting cells off plates
S.K.Green
- Drying sequencing gels
Petur Henry Petersen
- Expand PCR and TA cloning kits?
John Dixon
- SDS in western blot transfert
Dr E. Buxbaum
- primer extension
EFDG
- test message..
Osama
- murine oocyte cDNA library
STUTZ Andre
- Do you know?
Kurt Sims
- New Company WWW sites
martin LEACH
- SOS Call to all experinced "Sequencers"
Jim
- Soluble Protein with pEt
Tina Hallis
- RNase protections assays. Too much salt?
Jim
- Mycoplasma detection kits, and mycoplasma effects
Karen Achanzar
- Linker add., PCR amplif., cloning, seq'ng -> degraded R.E. sites?
Pete Muriana
- plasmid cDNA library amplification
TJ Murphy
- Any Problems With Oligo's??
Ted M.
- plasmid cDNA library amplification
Maureen_L_Ho
- TNT kit with T7 RNA Pol
Bernard Murray
- A Budded Yeast: One or Two?
Hernan Espinoza
- Company that makes long polyd[T]?
Robert SCHROCK
- Looking for TM Analytic
okada at ouvaxa.cats.ohiou.edu
- saturated ammonium acetate
Shanthi Adimoolam
- Linker add., PCR amplif., cloning, seq'ng -> degraded R.E. sites?
Sailesh Surapureddi
- cDNA cloning using expression library
Takahiko Ito
- primer extension
Ken Howe
- Culture of E.Coli (SURE) / Large Plasmids / Foreign Sequences
Joan Shields
- A Budded Yeast: One or Two?
Ken Howe
- saturated ammonium acetate
Shanthi Adimoolam
- saturated ammonium acetate
Sailesh Surapureddi
- SOS Call to all experinced
Daniel Pereira
- Gene Runner Manual:HELP!
Lois Johnson
- HELP! Proteins precipitates after glutathione column
Michael DiDonato
- oligo annealing/cloning problem
martin LEACH
- Peptide Nucleic Acids (PNA)
martin LEACH
- Linker add., PCR amplif., cloning, seq'ng -> degraded R.E. sites?
Pete Muriana
- Peptide Nucleic Acids (PNA)
Keld Sorensen
- Wanted: Peptide quantitation method
Keld Sorensen
- saturated ammonium acetate
Sailesh Surapureddi
- proteinase pretreatment???
Chi-Kuang Wen
- proteinase pre-treatment
Chi-Kuang Wen
- TNT kit with T7 RNA Pol
Mark Guiltinan
- Introducing silent restriction sites
Jen Logan
- faint PCR bands
rlee
- Automated sequencing reactions
Ed Castro
- RACE
vilimf01 at mcrcr6.med.nyu.edu
- MAP and Jun kinase assays
David Tak Yan Yu
- MAP and Jun kinase assays
Seth Corey
- Culture of E.Coli (SURE) / Large Plasmids / Foreign Sequences
Michael Koch
- Help with DNA extractions
Jimmy Rail
- PC-GENE program
Lee Daesang
- email addr. at Univ. of Bielefeld (Germany)
lonnie
- RNA-binding proteins
krafft at uni-muenster.de
- Culture of E.Coli (SURE) / better areation / Tunair Flaxk
WSchick at aol.com
- Peptide Nucleic Acids (PNA)
WSchick at aol.com
- primer extension
David L. Haviland, Ph.D.
- Help: Ribonuclease protection assays.
David L. Haviland, Ph.D.
- how to get rid of ethidium bromide???
P. Mertens
- Pellet paint ?
Nigel Kenward
- Getting cells off plates
Harold Drabkin
- In Vivo Excision
Seema Desai
- thermo-sequenase question
Sultan Kalifa
- calyculin A
Annick Breton
- PCR off membranes
varyc.mmcri at mmc.org
- USB Address
john brennand
- New Company WWW sites
martin LEACH
- RACE
Klaus Salger
- protein purification
RAFAEL ALDABE
- Competent cells, Inoue method - help!
Jeremy Marcus
- ANNOUNCE: EMBO Workshop on Molecular Biology of DNA Replication
Ulrich Hubscher
- Cutting Qiagen miniprebs??
Tracy Aquilla
- Rasmol crystal structures??
Mr. G. Morley
- macrophage electroporation
Michail Belyavskyi
- AvrII and StuI cutting peculiarities?
Robert Kraft
- SOS Call to all experinced "Sequencers"
Pierre-Alain Menoud
- Protein co-expression
Fred Hughson
- N-acetyl-galactosaminolactone source?
Peter Wang
- Siliconized coverslips for In Situ Hybridization
MPBELL at MVAX.hawaii.eduaccept
- EtBr staining acrylamide gels
David S. Guttman
- Repetitive Northern Hybridization
MPBELL at MVAX.hawaii.eduaccept
- molecular biology
Mr D. Kim
- Northern blots without formaldehyde
ruth e.w. walker
- Help about blood storage please!
ruth e.w. walker
- Heater parts for a Glass Still
Eric B. Carstens
- Subcloning sal1 insert into pBluescript
KHUANG at CHEMVX.TAMU.EDU
- SOS!--PCR restriction cut
Hector Cruz-Lopez
- saturated ammonium acetate
nsaunders at molbiol.ox.ac.uk
- saturated ammonium acetate
Sailesh Surapureddi
- BIAcore, IAsys, Westerns
Sanjay Krishnaswamy
- Incorporation of BrdU
Tom Phelan
- desferrithiocin
Scott Selby Med-Hem
- LCR (Ligase Chain Reaction) kit
Paul Ulanch
- Stripping W. blot membrane
MALINI at JASON.UTHCT.EDU
- Extra Long PCR reamplification
Russell Floyd Watkins
- WST-1,MMT assay cell viability: who knows?
Ed Castro
- HMG-CoA reductase
Daniel Tsi at Chung Li-hao
- Ligation
Royal Botanic Gardens
- mitochondrial dna
Mr Vid Mohan-Ram
- ANNOUNCEMENT: Understanding Protein Structure Determination
Lennart Nilsson (Karolinska institutet)
- Quiax-DNA-gel-extraction
Rico Laage
- Correction factor for spectrophotometrical quantitation of DNA
Cecilia Díaz Pohl
- Sequence Analysis Software
vipond at BSA.BRIS.AC.UK
- TAE vs. TBE?
Scott Hodgson
- Correction factor for spectrophotometrical quantitation of DNA
john brennand
- cfos and cun
David Tak Yan Yu
- Heater parts for a Glass Still
WSchick at aol.com
- PCR problem - disappearing bands
KUN Hematol.Lab.
- (none)
Shou-Jiang Gao
- Fixation of sequencing gels
GILLIAN NI BHARLO BOTANY PG
- PDB uk mirror sites
Mr. G. Morley
- 2D gels of basic proteins
Dr. Peter Friedhoff
- [Na] and Tm of oligos...
Bill Burnett
- thiamine concentration
Marcel Hoffmann
- thiamine concentration
Marcel Hoffmann
- thiamine concentration
Marcel Hoffmann
- (no subject)
Marcel Hoffmann
- thiamine
Marcel Hoffmann
- Cutting Qiagen miniprebs??
M.E. Hudson
- Searching for a CAT antibody !
Th.Neumann
- Getting cells off plates
martin LEACH
- searching for scientist's e.mail address
sl3wx at cc.usu.edu
- Rat cancer cell lines
Gil
- Wanted: Peptide quantitation method
Geoffrey D. Wheelock
- Blocking Chloroplast RNA
Edward Doran
- Siliconized coverslips for In Situ Hybridization
Imgenfm
- license for TAQ
Michael Cooley
- searching for scientist's e.mail address
Sailesh Surapureddi
- uncut vector removal...
rhubner at molbiol.ox.ac.uk
- firefly luciferase
Michael Cooley
- none
Jinny Lin
- Expression libraries wanted (HeLa and Brain)
Zhonglin Chai
- 125-I-Activin
Katri Koli
- uncut vector removal...
Michael Koch
- cfos and cun
Adrian.Philbey at SMTPGWY.AGRIC.NSW.GOV.AU
- Screning of a plasmid cDNA library
Yves HATZFELD
- HIV p24 Antigen
EKERRIGNEN
- HB-4 rotor
Leszek Andrzej Kleczkowski
- Specific transcription inhibitor
Stephane Ory
- protocol for mRNA extraction from E. coli
xiaoming yi
- Best inducible mammalian expression vector
Lisa Chakrabarti
- promoter active in both gram+/- bacteria
Moens William
- DHEA receptor
Nick Hunt
- Automated RNA isolation needed
books at mcvax2.d48.lilly.com
- thiamine concentration
Richard B. Taylor
- Delta Fingerprinting
David Tak Yan Yu
- Removal of unused primers from PCR rxn?
Mary P. Remington
- PCR optimization kits?
Mary P. Remington
- Building a UV-transilluminator
Alan Friedman
- promoter deletions
JMARTINEZ at AZCC.ARIZONA.EDU
- Screening for DNA binding protein
Daniel Pereira
- Post-doctoral position
Rajesh K. Mehra
- cloning up to 12Kb into a eukaryotic expression vector, needing RNA transcription only
Andrew Brooks
- Cloning up to 12kb into a eukaryotic expression vector, needing RNA transcription only.
Andrew Brooks
- Tg mouse/rat discussion group
Ed Castro
- promoter deletions
Dilip Dias
- Hygromycin:Help!!!!
Susan Hubchak
- Help with Plasmids and Strains Database?
Scott K. Boyer
- SOS Call to all experinced "Sequencers"
Leslie Jones
- pAlter mutagenesis
Matthew D Jonsen
- DHEA Receptor
Howard Fox
- Hygromycin:Help!!!!
kim myung jong
- Rep (1) : Any Problems With Oligo? s??
Mr SHIRE David
- DNA/RNA extraction kits
Sandrine Imbeaud
- DNA EXTRACTION FROM CHLAMYDIA OR MYCOPLASMA
Julie Logan
- Re- removal of unused prime
Brett Beitzel
- Corynebacterium media/habitat?
R.A. Skuce
- Rep (3) : Any Problems With Oligo? s??
Mr SHIRE David
- license for TAQ
Song-Kun Shyue
- Help! Image process software need!
Song-Kun Shyue
- Glucose phosphate isomerase
Dr. Eric Bouhassira
- Molecular Probes Web site
ICleme1659
- 125-I-Activin
S.
- "Differetial Dismay" reference
ege
- GST-Fusion protein proteolysis
Brian J Murphy
- ELISA plate washers
Brian J Murphy
- Celery cDNA wanted
Daniel Solaiman
- HOMEMADE MERLIN GEL PURIFICATION
Chanda Eva R
- HOMEMADE MERLIN GEL PURIFICATION
martin LEACH
- Differential Display
Tonja Burshek
- HOMEMADE MERLIN GEL PURIFICATION
Paul N Hengen
- "Differetial Dismay" reference
Shelley Cole
- Pellet paint ?
Paul N Hengen
- Second Electronic Glycoscience Conference
Iain Wilson
- Soluble Protein with pEt-Sorbitol/Betaine
Bradley Turner
- cloning up to 12 kb into mammalian cells
Andrew Brooks
- cloning up to 12 kb into mammalian cells
Andrew Brooks
- cloning up to 12 kb into mammalian cells
Andrew Brooks
- test
mook
- Glucose phosphate isomerase
Arle Kruckeberg
- Needed: Protein that ppts at less than 25% NH4SO4
Randy Rasmussen
- PVDF: Total protein stain & scanning
Arle Kruckeberg
- Luciferase mRNA stabilize
FANX0 at BIOMED.MED.YALE.EDU
- which company sells plant poly(A) RNA?
Jeremy Chen
- Pellet paint ?
martin LEACH
- molecule
sebastien bergeron
- license for TAQ
WSchick at aol.com
- (no subject)
Eric Hanson
- Alternative to autoclaving for denucleasing plasticware
Wayne Coco
- ISCO and Sartorrius email addresses
Andrey Chernov
- mystery band pCDNA3/EcoRI/-PO4
Frances Hannan Zoology
- partial cleavage by Thrombin
sl3wx at cc.usu.edu
- Tyrosine phosphatase inhibitor ?
Cyril Gueydan
- random priming
Lely Chow
- Cutting Qiagen miniprebs??
QIAGEN
- phage display antibody
Clemens Gillen
- Strange smile
Roland Koelliker
- PVDF: Total protein stain & scanning
logan
- Strange smile
Alexander Kraev
- PCR from fixed tissue
Catherine Peichel
- DNA laddering (apoptosis)
Dr. Simmen's Lab
- Sequencing Services
Besharse Lab3
- abi-autoseq problems
aaron jacob kelly
- Conjugated luciferase
Donald Lo
- 3' UTRs Intact?
Robert A. Schoenfeld
- How to get rid of Ethidium Bromide
Helen McBride
- fluorometer or spectrofluorometer, who sells 'em
Robert Roxby
- Centrifuging MO's
Bunny Lover
- WST-1,MMT assay cell viability: who knows?
Bradley Turner
- Oligo price wars?
Jody K. Hirsh
- fluorometer or spectrofluorometer, who sells 'em Turner Designs
WSchick at aol.com
- Plasmid Reference Center
Castor Menendez
- RNA-binding protein
krafft at uni-muenster.de
- Help with Electroporation!!!
Peter
- DNA sequencing service survey
Dennis Templeton
- Northwestern
Joachim Kremerskothen
- Help wanted, antibiotic cassettes
Jackie Fetherston
- DNA sequencing service survey
Seth Corey
- RNA isolation
Paul Chien
- Cytokines Web
Simon M. Brocklehurst
- scFv cloning and expression - problem with pseudogenes
Regina Shaw
- PCR enzymes
Tori
- Test - please ignore
john brennand
- DNA isolation protocol
Blanca Lupiani
- HA Tag cross reactivity
L. Fliegel
- RACE/Anchored PCR
Anil K Goklaney
- killer gene in yeast
Ray Lu
- DNA purification from gram-positives question
Igor Sidorenkov
- scFv cloning and expression - problem with pseudogenes
RVDESHP0 at pwinet.upj.com
- Help with Electroporation!!!
tera
- Help with Electroporation!!!
tera
- Finding the whole sequence . . .
Todd Griffith
- alternative to wedge sequencing gels
Peggy Halleck
- Re- amp cassette
Brett Beitzel
- *** CAN ANYONE EXPLAIN THIS????***
Dr Mark Watson
- Cloning problems with nls-lacZ
Martin Spiegel
- Weird, bizzare, inexplicable sequencing problem
Sarven Sabunciyan
- GenePulser
Masato Kinoshita
- RNA isolation
Sarven Sabunciyan
- NIH grant templates for Mac Word 5 and Excel available
Dennis Templeton
- mtDNA primers - Call for Marathon!!!
Benny Shomer
- can normal peripheral B cells be transfected ?
fouret
- weird, strange and inexplicable membrane proteins recovery.
mazella at naxos.unice.fr
- random primed directional cDNA library
xxxxxxx
- DNA sequencing service
M.Sauerborn
- human c-rel & p65
Poul Sorensen
- Protein blocking solution for DNA hybridization
S. Macfarlane
- Protein blocking solution for DNA hybridization
S. Macfarlane
- how to get rid of ethidium bromide???
john haynie
- inosine in oligonucleotides
Robert H. Proctor
- kanamycin
Jan Paul Bebelman
- Avidin-Biotin Reaction
JohnnyHugh
- How to crack yeast for PCR ??
Michael Liss t4310
- Need: Protocol for Dig-labbelling PCR
Petur Henry Petersen
- cDNA First strand synthesis
Philip Heacock
- mtDNA
Scott Selby Med-Hem
- Need: Protocol for Dig-labbelling PCR
$user
- Avidin-Biotin Reaction
Keld Sorensen
- help with ligation
Victor Yankiwski
- archive search engine?
davesmith
- Help: 2 Plasmid Electroporation /Infection
TJ
- help with ligation
Edgar Valencia
- Northerns of large RNA?
Kay A. Pleyte
- How to crack yeast for PCR ??
Jeremy Marcus
- PCR hood
Pamela J Snyderer
- Electroporation Cuvettes
Eurie Lee
- tearing my hair out over IPs
Jen Logan
- Fluorography for 35s labelled proteins
Mr Nicholas Moody
- EtBr staining acrylamide gels
AShaw5
- Linear acrylamide (was --Re: glycogen as a carrier)
AShaw5
- test mail please ignore
Ruud Ross
- Culture of E.Coli (SURE) / Large Plasmids / Foreign Sequences
Chris Boyd
- cosmid amplification
vdilbeck at dbm.ulb.ac.be
- protein blocking of DNA/RNA hybridisations
S. Macfarlane
- protein blocking of DNA/RNA hybridisations
Ned Mantei
- protein blocking of DNA/RNA hybridisations
Joao Vasconcelos Costa
- Address for Biocarb company
frosc000 at mzdmza.zdv.uni-mainz.de
- Gene synthesis using oligos
Dr. Duncan Clark
- this is a testmail please ignore
Ruud Ross
- Gene synthesis using oligos
mike Watson
- RNA Oligo supplier???
Andrew Cunningham
- Fluorography for 35s labelled proteins
HIRANYA ROYCHOWDHURY
- Reporter Assays
Elonex3 at umds.ac.uk
- How to advertise PhD
Michael Robert Ladomery
- protein blocking of DNA/RNA hybridisations
Brian A. Flannigan
- FW: How to crack yeast for PCR ??
Stewart, Robert
- Problems in protein solubilization
Huseyin Kucuktas
- Transfection of L and Clone 9 Cells
Jason R. Dyck
- Chicken antibody purification
Alex Abbas
- help with ligation
Mr. Chris Hoyle
- DEAE-transfection of EL-4 cells
Paul D. Boyer, Ph.D.
- Reporter Assays
joestudent at mit.edu
- trizol
GE Schwab
- Green Fluorecent Protein gene for Reporter
Toshio
- How to crack yeast for PCR ??
Mark Prescott
- In situ PCR - mtDNA
Jim Cummins
- IMPORTANT - Sun Microsystems and Academic Press sponsor BIOSCI!!
BIOSCI Administrator
- testing mail to methods@daresbury.ac.uk,please ignore
Ruud Ross
- MW of mouse IgG and protein A in IP
Tang Hsin Yao
- NdeI: cutting problems?? anyone else?? seems silly! (fwd)
PRETORIUS
- ABI 373A Read Length Improvements
Mick Jones
- PCR Mutagenesis
Jacques OBERTO
- Fermenter
Andrei Serban
- LexA
Rudi Beyaert
- Does drying of PVDF membrane help immunodetection?
G.P.
- Nonradioactive screening systems
Julie Drawbridge
- Single primer cloning
KHUANG at CHEMVX.TAMU.EDU
- cloning amplicons with inosine
David Demezas
- PCR hood
upyers
- Library screening with oligos
Tanya Reichert
- cDNA suddnly smaller??!!
Peter
- Does drying of PVDF membrane help immunodetection?
Keld Sorensen
- Native Gel Difficulty
nc26 at wwa.com
- unsubscribe
Noriaki Tamura
- denaturing vs. non-denaturing electrophoresis
Shanthi Adimoolam
- GENE REARRANGEMENT PROBES?
kevin gilbride
- Randomising aa's, how?
Troels Wind
- help on Moab-enzime linkage (for ADC)
Rodrigo Cassio Sala Veneziani
- Miniprep SNAFU
Hideously Confused
- Help - yeast plasmid rapid miniprep needed!
Stephan Witte
- PCR
Osama
- Sequence analysis programs for Linux
Stephen P Lee
- calculation of % identity & % similarity in pairs of seq
Jaishree Muppala Chittoor
- DNase cuts
luis brieba
- DNA extn from high phenolic tissue?
Michael Adamson
- ribosomal RNA in DD
Markku Vaarala
- fluorescent oligonucleotide source
Daniel Jacobs
- Knock-out of bacterial gene
Jose M. Bautista
- Re- DNase cuts
Brett Beitzel
- Sequencing Problems w/ Prism SEQUENASE terminator/abi system
Jimbo Jones
- Correction to prism sequenase inquiry
Jimbo Jones
- Radiolabelling proteins in Coli
john brennand
- Miniprep SNAFU
john brennand
- ANNOUNCEMENT (ETRO Working Party on genetics and vascular
diseases)
Francesco Zito
- Random Hexamer PCR
Brett Beitzel
- RNase denaturing after protection
Jiri Forejt
- (none)
Szabadkai Gyorgy
- Double Helix
Gerardo Medrano
- Overexpression of 2 genes from the same vector
Robert Hindges
- RNA isolation from whole organism rather than tissue or cells
john yon
- Gibco Stbl2 cells: deletion curse!
walter
- Terminal transferase and sequencing
Dr. Jeff Lightfoot
- DNA extn from high phenolic tissue
Michelle Gleeson
- pTAQ licence
KHUANG at CHEMVX.TAMU.EDU
- condition of Electroporation
Masato Kinoshita
- Labelled oligo's for the LiCor sequencer
Your Name
- SuperSignal - Ultra sensitivity
Mr Blue101
- Miniprep SNAFU
Wei Hu
- [solubilization of inclusion bodies with SDS?]
Tan Nguan Soon
- Nonreducing Gel
nc26 at wwa.com
- Knock-out of bacterial gene
Tom Bickle
- test mail please ignore
Ruud Ross
- Short oligo probes for Northerns ??
Patrick HJ Falckh
- TECAN-Genesis robot
Glibert Fabrice
- snail genomic extraction
pdxrmb at vaxa.nott.ac.uk
- ANNOUNCE: High quality, low price Fmoc-amino acids from ALEXIS
Dr. Stephen Hoare
- !!!!!! M-CSF (CSF1) PROMOTER PCR PROBLEMS !!!!!!!!
MINET Emmanuel
- help? info re SDS effects
Juliewoods
- mg+2/ca+2 atpase
Mr Vid Mohan-Ram
- sds effect on tissue storage
julie feinstein
- DNA extraction from formalin-fixed brain tissue
P.Orange
- Native gel
Shanthi Adimoolam
- TimeSaver cDNA Synthesis Kit
Stefan Kappeler
- !!!!!! M-CSF (CSF1) PROMOTER PCR PROBLEMS !!!!!!!!
Heinz-Juergen Schaefers
- E. coli XL1-Blue MR strain - question!
Dan Kearns
- E. coli XL1 Blue MR follow up
Dan Kearns
- E. coli XL1 Blue MR follow up
Mikhail Alexeyev
- cyclo sequencing
Joan Shields
- E. coli XL1-Blue MR strain - question!
Michael Benedik
- phage display library
sujoy ghosh
- 80/Base
Jill Kellogg
- CTAB method of DNA purification
Eva Pietrzak
- hybridization question
Eva Pietrzak
- Pouring sequencing gels the old fashion way
Rob Jordan
- Help Needed with Library Screening
jchristn at umich.edu
- reptile antibody purification
Ellen.Ariel at jcu.edu.au
- Timesaver cDNA synthesis Kit
KHUANG at CHEMVX.TAMU.EDU
- Difficult transfert in western blot
Giorgio Spagnol
- Are plasmids purified by Qiagen really that bad?????
The Wagner Lab
- conserved 5'UTR
D
- Human cDNA expression library wanted.
Dr Zhonglin Chai
- test, please ignore
Jun Yasuda
- Betagen address
Jun Yasuda
- PCR patent debate - part 73
john brennand
- ***ADVICE ON VIRAL RNA PREPARATION PLEASE!*****
Dr Mark Watson
- Long Range PCR Question
KONSTANTIN.KANYUKA at bbsrc.ac.uk
- Reply to RNA ISOLATION FROM WHOLE ORGANISM
Gerardo Medrano
- Corynebacteria media and habitat?
R.A. Skuce
- FROZEN acrylamide sol'n!
Timothy R Jackson
- Corynebacteria media and habitat?
Mark Fuller
- PEI transfection
Philippe Lefebvre
- **HELP!** Binary Vector Ligations
Yolande Knight
- BL21-K12?
crbaker
- Restriction enzyme digest
sujoy ghosh
- Tc inducible system
Danny Altshuler
- Are plasmids purified by Qiagen really that bad?????
Sailesh Surapureddi
- Biotinylation of MoAbs
Mick N. Mulders
- Pouring sequencing gels the old fashion way
Sailesh Surapureddi
- pouring gels the old way
Jillian Johnston
- Unusual start codons
Richard Hastings
- High G-C PCR...help.
Steven James Carlisle
- S1 Nuclease
chip peele
- Good inhibitor to RNAses?
Idur
- Molecular Probes Web Page
Computer
- replicator
DAB at CNSVAX.ALBANY.EDU
- pComb3H/Fab library QUESTION?
X
- replicator
Sailesh Surapureddi
- Baculovirus
Crouse, Joe
- test please ignore
alan kong
- Vector Laboratories, CA - address/tel. # wanted
Simon Twigger
- Avidin-Biotin Reaction
madbrow
- Exonuclease deletion kits...help !!!
Sensational Gravity Boy
- Stability Bead-Avidin-Biotin-DNA?
James Graham
- Electro-transformation of mammalian cells
Heinz-Juergen Schaefers
- looking for plasmids pTrcHis A,B,C (Invitrogen)
CAMPEAU,ERIC,M
- electroporator
Pak-Lam Yu
- SuperSignal - Ultra sen
Brett Beitzel
- Biotinylation of MoAbs
David L. Haviland, Ph.D.
- Restriction enzyme digest
HIRANYA ROYCHOWDHURY
- Vector Laboratories, CA - address/tel. # wanted
Simon Twigger
- lipoproteins/colloid gold labeling
N.P.Dantuma at biol.ruu.nl
- Are plasmids purified by Qiagen really that bad?????
Sailesh Surapureddi
- oligo synthesizer
Linkman
- Are plasmids purified by Qiagen really that bad?????
Seng-Lai Tan
- The lower limits of hygromycin
Haunani A. Soares
- Plasmids purified by Qiagen - just fine!
Nikolai Chitaev
- **HELP!** Binary Vector Ligations
Nikolai Chitaev
- REQ: rat interleukin-6 antibody
Luc Vallières
- pTAQ licence
KHUANG at CHEMVX.TAMU.EDU
- Problems with ABI sequencer. Help !!!!!
Keun-Hang Yang
- Electrophoration for lymphoma cell Molt-4
Masato Kinoshita
- Chloroform & lambda: How and why?
acatalan at EXTRO.UCC.SU.OZ.AU
- Qiagen Maxi: Quantity
Gerd Romanowski
- real-time advice
Mr Vid Mohan-Ram
- Restriction digest software
Peter Russell
- (no subject)
Shaun Heaphy
- Qiagen Maxi: Quantity
john brennand
- electroporation with HEK
Deslauriers Benoit
- Vector Laboratories, CA - address/tel. # wanted
jamesm at holyrood.ed.ac.uk
- lowest hygromycin concentration
Andrei Popov
- Retic. lysate for degradation?
Matthias Zeiner
- TA cloning
Blanca Lupiani
- test ignore
tflanigan at molbiol.ox.ac.uk
- Precipitation of DNA/RNA with isopropanol
Stein Tore Solem
- OD ratios of 3 to 4!?
tflanigan at molbiol.ox.ac.uk
- (none)
Pratima Nangia-Makker
- Precipitation of DNA/RNA with isopropanol
john brennand
- PCR Patent Debate
Brett Beitzel
- OD ratios of 3 to 4!?
john brennand
- uncatalyzed phospholipase-D reaction
phillip messersmith
- TA cloning
Paul N Hengen
- Review Antibody Production
lena brydon
- real-time advice
John MacFarlane
- TA cloning
KHUANG at CHEMVX.TAMU.EDU
- How many bases can be read with ALF-DNA-sequencers, who was good results with other automatic DNA-sequencers?
Ruud Ross
- 2DSDSPAGE STNDS
Elke
- ECL on NC
Freek
- (none)
Ming Fa
- DH5alpha E coli cells
Elli Wurtzel
- Spin-label probe and distance measurement
Ming Fa
- **HELP!** Binary Vector Ligations
Jill Kellogg
- Precipitation of DNA/RNA with isopropanol
Eric C. Anderson
- pZeoSV map, Help Help ASAP
tamakoto Chang
- DNA extn from high phenolic tissue?
David C. Logan
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Tonja Burshek
- How many bases can be read with ALF-DNA-sequencers, who was good results with other automatic DNA-sequencers?
Jose M. Bautista
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David C. Logan
- Sequencing phage DNA directly
KHUANG at CHEMVX.TAMU.EDU
- DNA
Hyder Ali Khan
- testing
ADN Computer Center Public Labs
- testing
ADN Computer Center Public Labs
- The lower limits of hygromycin
Dima Klenchin
- test-please ignore
michael_weiner at glaxo.com
- differential display PCR
Renee Trudeau
- slide printer
Bob Gross
- NdeI cutting BIG HUMBLE APOLIGY
gerry oneill
- HELP! 3-way ligation
Maria C. Costanzo
- Ligations with 1 cohesive & 1 blunt end...best conditions?
Kathy L
- Sf9 cDNA library
Mark Vickers
- Sf9 cDNA library
Mark Vickers
- pZeoSV map, Help Help ASAP
Bernard Murray
- Sequencing phage DNA directly
John Ladasky
- RNA quantification
Shelley Cole
- Trizol RNA purity
Edward Wang
- GFAP immunofluoresence of C6 cell line
Ari Kahn
- Ligations with 1 cohesive & 1 blunt end...best conditions?
Nikolai Chitaev
- DOWNTIME - Friday 11PM to Sat 6PM PDT
BIOSCI Administrator
- Purifiction of total nucleic acid and protein. How??
Ratner Lab
- Expression libraries wanted (HeLa and Brain)
Dr Zhonglin Chai
- How many bases can be read with ALF-DNA-sequencers, who was good results wit
WSchick at aol.com
- EtBr staining acrylamide gels/with sample
WSchick at aol.com
- Rep : oligo synthesizer
Mr SHIRE David
- BIOTIN - is it still active?
Ming Fa
- Peptide transfer to PDVF
Ming Fa
- BIOTIN - is it still active?
Malgorzata Wilczynska
- Peptide transfer to PDVF
Malgorzata Wilczynska
- Looking for 35S-APS
Jose F Gutierrez Marcos
- (no subject)
Philippe Lefebvre
- pcr licenses
John Wise
- Role of serine in smooth muscle contraction
Suleyman
- Suramin (Michael)
glycotech
- Extraction of DNA from Soil Samples
Xiang Li
- Adress Berkeley Antibody
Eyck Poetter
- Suramin (Michael)
john brennand
- Suramin (Michael)
john brennand
- (none)
Coffman, Dan
- Is anyone sequencing with the Prism Sequenase DS Template Kit (w/abi system)
Jimbo Jones
- antibodies to K channel subunits?
tflanigan at molbiol.ox.ac.uk
- VTWB PCR Machine??
Gene Huh
- Quinol
chan at bioch.ox.ac.uk
- ABI 373a, sequencing, and FMC LongRanger gel
R.Tate
- ignore this
CKY at mail.nerc-oxford.ac.uk
- POINTER TO FAQ LIST
Paul N Hengen
- testing mail
CKY at mail.nerc-oxford.ac.uk
- Help Address Sarasep Inc
Eyck Poetter
- Chemiluminescent Detection Kits
Paul N Hengen
- Suramin source
arturo.galvani at itner.pharmacia.se
- help with semiQ RT-PCR
Janet E. Rubin
- Cytochrome c extraction
Ron Caspi
- Marathon/RACE Experiences?
Bradley Turner
- mRNA isolation - your recommendation?
Dr. Victor V. Levenson
- Anti-Human Cytokine X-Reacts with Simian Cytokines?
upyers
- Quantification beta-glucan content
Gabriela Martinez Trevi o
- pKa of SDS
HIRANYA ROYCHOWDHURY
- help with semiQ RT-PCR
Bill Alexander
- Trizol RNA Purity
Gerardo Medrano
- Rep : oligo synthesizer
natalie unruh
- CTAB....
Anonymous
- slide printer
Ron Caspi
- PCR of cDNA library
Mitch Lazar
- ANNOUNCE: High quality, low price Fmoc-amino acids
Jill Kellogg
- buffer pH and temperature
Enrique Jose Labadan Frio - SCBC - 3421101
- About ligation
KHUANG at CHEMVX.TAMU.EDU
- Help: Endocrinology
Eyck Poetter
- blunt&sticky-1 Ligation?
Mark Henteleff
- Agbiotech Conference Saskatoon (fwd)
lees at SASK.USASK.CA
- Methyl Mercury Hydroxide
Administrador de iihema.fmed.uba.ar
- Ni-NTA Spin Columns
Neil Harris
- Staged Embryos
Martin Cann
- Speed cruise web bioresources
Cynthia S. Smagula
- EtBr and polyacrilamide gels
Ed Kennedy
- Rep : oligo synthesizer
WSchick at aol.com
- RAPD PCR primer for Trout?
Raymond Deitz
- HELP - RT-PCR
David Webb
- Help with high mol wt protein stds
Vidhya Rangaswamy
- DNA EXTRACTION.
Dale Levitzke
- Quantitative RT-PCR
Paul Mozdziak
- test, please ignore
Markus Ruf
- RNA Molecular Weight Marker
Markus Ruf
- **HELP!** Plant growth medium contamination
T. Hedley Carr
- Help with high mol wt protein stds
Sailesh Surapureddi
- Methyl Mercury Hydroxide--Supplier
Bradley Turner
- Marathon-Ready cDNAs from Clontech
Todd Griffith
- Rep : Quantitative RT-PCR
Mr SHIRE David
- Linear acrylamide (was --Re: glycogen as a carrier)
Paul N Hengen
- Protein 2d structure
Michael Mair
- Rep : Quantitative RT-PCR
Mr SHIRE David
- help with northern quantitation!
Richard Converse
- help with a c-myc tagging vector?
Richard Converse
- **HELP!** Plant growth medium contamination
David C. Logan
- de-lipidization
Helen Korsmo
- GST and Glutathione Sepharose 4B
Tanel Pungas
- ABI 377 question
Kevin Munnelly
- de-lipidization
brett
- bodipy, tamera, cy5
Michael Glass
- Plasmids purified by Qiagen - just fine!
Nikolai Chitaev
- 5'RACE kit
Gabriel Romero
- Asymmetric PCR
Doug Johnson
- random primed cDNA cloning kit
DAB at CNSVAX.ALBANY.EDU
- Magnetic Particles for Separation in Biosciences and as Drug carrier
Mueller-Schulte
- NEW technologies for affinity chromatography, immunoadsorbents, biocompatible polymers!
Mueller-Schulte
- Hormones to Antibodies?
Kai Lewis
- Vector as cDNA primer??
James Graham
- Not sizing cDNA (random primed)?
James Graham
- hybing without shaking??
Lely Chow
- Hydrotech vacuum pump
harvey
- southern blot lower limit
elida at parasit.lan.mcgill.ca
- Ceramide measuring
Administrador de iihema.fmed.uba.ar
- Linear acrylamide (was --Re: glycogen as a carrier)
(Dave Johnston)
- DNA in ELISA's
Michael Adamson
- HELP - Frrze-drying of Lymphocytes.
G K Sharma
- Hydrotech vacuum pump
WSchick at aol.com
- ABI 373a, sequencing, and FMC LongRanger gel
STEVEN GERHARDT
- Nuclear Run-ON Protocol
Jimbo Jones
- southern blot lower limit
Michael W. Thompson
- Capillary transfer from polyacrylamide-gels
Thomas Döring
- Linear acrylamide (was --Re: glycogen as a carrier)
Paul N Hengen
- RNA quantification
Shelley Cole
- Gel Imaging Systems
Greg Schaefer
- Linear acrylamide (was --Re: glycogen as a carrier) SIZE??
Bradley Turner
- Hormones to Antibodies?
Michelle L. Leonce
- poly-His Ab
Seth Corey
- Hirt extracts
Ian A. York
- Fas, Bcl-2, sgp-2 probes: where?
Ed Castro
- reproduciblity silver staining in native gradient gels
P.H.A. Quax
- Clontech's "Capfinder PCR cDNA library construction kit": Does it work?
Andrew Lange
- For all you, electroporation fans!
Jfloring at aol.com
- Betagen address
WSchick at aol.com
- Another Ligation Problem
Carolyn Calnan
- DNA EXTRACTION.
WSchick at aol.com
- IMPORTANT - BIOSCI Fundraising Update!
BIOSCI Administrator
- LacSwitch
Andrea LeBlanc
- Gene Expression and mRNA stability
Osama
- Quantitative RT-PCR on blood
P. Mertens
- exon amplification/trapping
Rachelle Donn
- Expression of EGF receptor cytoplasmic domain
zhixiang Hu
- "Four-color single lane" references
Mark E. Berres
- In Situ HELP
Chris Regan
- LOOKING FOR GLUTAMATE RECEPTOR PROTEIN expressing gene...
Matthew J Vona
- transcriptional activity and mRNA stability
Jimbo Jones
- DNA on a leash?
Tina Hallis
- Need Pure Histone-core
Matthew J Vona
- 2D Protein Gels
Mohanish at pharmdec.wustl.edu
- in vitro packaging of cosmid
Dr. Koen De Smet
- Subtractive Hybridization Pitfalls?
James Graham
- Glycogen as a carrier
KHUANG at CHEMVX.TAMU.EDU
- Globomycin
Christine Andrews
- Band-shift assay with U937
Ralph Melcher
- PCR diagnose technology
William Jia
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Brian Brunk
- Summary of "Pouring sequencing gels the old fashioned way" responses
Rob Jordan
- Why low second-strand cDNA?
James Graham
- SV40 LT purified proteins
Jorge MORENO
- TE degradation of DNA
Richard B. Taylor
- promoter analysis
ANNE GEORGE
- Marathon-ready cDNAs from Clontech
ANNE GEORGE
- RNA Molecular Weight Marker
K J C 944
- Brassinolide
Johannes Hanson
- shooting yourself in the foot....
Mr Vid Mohan-Ram
- PROGENE/TECHNE THERMOCYCLER
Jan Schröder
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Jan Schröder
- PROGENE/TECHNE THERMOCYCLER
Jan Schröder
- PROGENE/TECHNE THERMOCYCLER
Jan Schröder
- exciting bugs
Julian DiAvanti
- PROGENE/TECHNE THERMOCYCLER
Jan Schröder
- ** PROGENE/TECHNE THERMOCYCLERS **
Jan Schröder
- Klentaq DNA polymerase
pgt
- TE damage to DNA
Richard B. Taylor
- shooting yourself in the foot....
Bill Alexander
- PROGENE/TECHNE THERMOCYCLER
Richard J. Dudley
- Debugging ARMS PCR with internal control
John Ladasky
- Nested deletion: kits? - suggestions?
radomski
- Silver Thiosulfate???????????
Dr. Randal W. Giroux
- PCR FAQ
Edward Wang
- a mystry
Edward Wang
- XL1-Red in vivo mutagenesis
neuro
- Sending bacteria via mail
Georg Kroeger
- a mystry
K J C 944
- DNA Extraction from frozen mammalian tissue
bjh8 at Lehigh.EDU
- dynabeads
Anthony Martinez
- Desaturation of [9,10-H3]stearyl-CoA
Cary Stewart MSTP '98
- PCR FAQ
Paul N Hengen
- Qiagen Problem (secret buffers)
Paul Wakenight
- HSV1 TK antibody
PS Evans
- TE damage to DNA
Julie Drawbridge
- Lactococcus peptidoglycan prep.
lothar at lmb1.rug.ac.be
- Syntex protest
Altnews
- Klentaq DNA polymerase
Mike Ma
- Single error in Primer
Patrick HJ Falckh
- Silver Thiosulfate???????????
David C. Logan
- lipid mediated transfection of fish cells
kee ng
- Buffer calculation
Sabine Hanelt
- pK18 plasmid info
vilimf01 at mcrcr6.med.nyu.edu
- in situ pcr protocol for bacteria?
David Haydon
- Hemin detection
FALQUET Laurent
- FLAG-tag from Kodak
FALQUET Laurent
- cre-lox vectors cause recombination?
Shiao Y. Wang
- Comparison of thermocyclers
Jan Schroeder
- Growing Containers!!
awb at aber.ac.uk
- microwaves
jean
- DNA transcription
Susan Goldsworthy
- cDNA quantification
Myriam Onno
- uncut vector DNA
Shanthi Adimoolam
- Sequencing quotes needed
H.J. Sluiman
- Phospholipase B substrate (s) ?
Stephane Ory
- brain slices for Ab stain - company?
hong dang
- the best thermal cyclers
Marc Visconti
- Sequencing quotes needed
iddavis at vms.cis.pitt.edu
- uncut vector DNA
Mark R. Miglarese
- uncut vector DNA
Richard J. Dudley
- Thermo RT's & cDNA library (hi G+C)
James Graham
- uncut vector DNA
George Rutherford
- ability to pcr introns from cDNA
Nancy Duncan
- v-abl and phosphatase assay
annette walter
- Help: method of inactivation
Anatoly Sharipo
- (none)
Steve Schramm
- Obtention ribosomal subunits
Gabriela Martinez Trevi o
- obtention ribosomal subunits
Gabriela Martinez Trevi o
- Sending bacteria via mail
brett
- PROGENE/TECHNE THERMOCYCLER
WSchick at aol.com
- license for TAQ
WSchick at aol.com
- shooting yourself in the foot....
WSchick at aol.com
- peptide gel
gc
- unsubscribe
BOYDSTON at MVAXGR.HUNTER.CUNY.EDU
- Basic Info
Mr. J.B. Mohd Ali
- PCR-Mimics- construct kit-Internal Controls for Quantitative PCR
Paul Mozdziak
- Uncut vector DNA-again
Shanthi Adimoolam
- ability to pcr introns from cDNA
Robot Boy
- Uncut vector DNA-again
Sailesh Surapureddi
- Uncut vector DNA-again
Nikolai Chitaev
- unsubscribe
BOYDSTON at MVAXGR.HUNTER.CUNY.EDU
- Purification of antibody raised against hexahistidine tagged protein
Leo
- suspension culture
Mr Hassan Abdulrazzak
- Problem in PCR
mdbcs002 at CMU1.CHIANGMAI.AC.TH
- Mailing bacteria
Georg Kroeger
- Software for strain collections
Michael Kertesz
- Using collage to analyze blot
Monica Magidin
- I'm looking for Microplate Reader's info
Vladimir Yu. Kuryshev
- Help with DIG Northerns
Helen Haase
- MD: Molecular Biology Position Available
John L. Seed
- Expression libraries wanted
Dr Zhonglin Chai
- auditory nerve cells
Titta Malinen
- mitchondrial dna inheritance
Mr Vid Mohan-Ram
- Dichloromethane vs. Chloroform
acatalan at EXTRO.UCC.SU.OZ.AU
- Corynebacterium media/habitat?
R.A. Skuce
- About ligation
Robert Cowherd
- DNasing RNA samples
Pete
- ss vs ds DNA
bjorn eriksson
- Dichloromethane vs. Chloroform
Richard J. Dudley
- UNBLOT Protocol
GriffCouch
- Storm Imager 840
Tracy E. Anderson
- DNA precipitation by Polymine P
Jean Paul Raffin, Ifremer Brest PDG-DRO-EP
- Sooo has anyone found my mysterious paper yet?
DANIEL Y KIM
- luminol and luminometer
Robert Bateman
- cloning of YAC DNA
larsen at CALSHP.CALS.WISC.EDU
- "Motivation" for grad students
The Wagner Lab
- Obtention ribosomal subunits
Gabriela Martinez Trevi o
- T7 RNA Pol in vivo assay
luis brieba
- To Pre or not to Pre ?
Mr Hassan Abdulrazzak
- Western blots
olle vidal
- mitchondrial dna inheritance
Roy Manns
- CGH
Gizbab
- QuickChangeTM Mutagenesis
Daniel Durocher
- PCR Hell
Pam Snyder
- "Motivation" for grad students
brett
- Help with Clonetech
Leandro Peral Pontigo
- Blood spot DNA Extraction
John Christodoulou
- cutting plasmids for PCR
Rico Laage
- motivation of grad students
GILLIAN NI BHARLO BOTANY PG
- Commercial source for HA-probe antibodies????
Pramathesh S. Patel
- TMAC tetramethyl ammonium chloride
Steve Griffiths
- BIG PCR WITH TMAC
Steve Griffiths
- What is a good Conc. of DAPI to USE for F.I.S.H.??
dasuser at PO-Box.McGill.CA
- direct PCR from E. coli
Geraldine Butler
- Amazing PCR results with TMAC
Steve Griffiths
- direct PCR from E.coli
KHUANG at CHEMVX.TAMU.EDU
- PC12 cell line
Jane
- His-tag protein purification
Ian Goodfellow
- Cell lines for transfecting
IAN MCFARLANE
- Getting rid of Proteinase K?????
bjh8 at Lehigh.EDU
- Do A's really fall off over time?
John Dixon
- -80C Freezer
Mike Holbrook
- test-ignore
Don Faller
- What is Histidine tagging like?
DANIEL Y KIM
- "Motivation" & Attitude
brett
- peritoneal cell types
Lo Wing Ip Anthony
- -80C Freezer
Brian W. Tague
- peritoneal cell types
Ross S Feldberg
- anti darc antibody anti-Fy6
chris moore
- Tetanos toxin frag. C
lothar at lmb1.rug.ac.be
- peritoneal cell types
Edgar Valencia
- Plant Science Postdoctoral Position
Brandt Cassidy
- PCR to detect mycoplasma
David Tak Yan Yu
- help with cDNA.
aparjita ghosh
- Obtention ribosomal subunits
Gabriela Martinez Trevi o
- Do A's really fall off over time?
KHUANG at CHEMVX.TAMU.EDU
- Stratagene quick change mutagenesis
Bret Sellman
- LacSwitch
Display Systems Biotech
- need info on superantigens
Eva Pietrzak
- Northern Probe Size
Patrick HJ Falckh
- TAP 90 cells please?
Bart Janssen
Last message date:
Tue Apr 30 23:40:11 EST 1996
Archived on: Sun Mar 6 21:02:25 EST 2005
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