Removing endotoxin from plasmids
msklar at cts.com
Thu Aug 1 00:34:52 EST 1996
Hi. I'm hoping someone can help me out. I've been trying to purify
endotoxin free plasmid DNA for in vivo work using a variety of methods.
I've tried using various kits and Phenol/chloroform to clean it up and
nothing seems to work. My concentrations are still 2-5 EU/ug. Can
anyone offer a useful protocol or method for cleaning up the DNA? I'd
really appreciate it.
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