Removing endotoxin from plasmids

Mike Sklar msklar at
Thu Aug 1 00:34:52 EST 1996

     Hi.  I'm hoping someone can help me out.  I've been trying to purify
endotoxin free plasmid DNA for in vivo work using a variety of methods. 
I've tried using various kits and Phenol/chloroform to clean it up and
nothing seems to work.  My  concentrations are still 2-5 EU/ug.  Can
anyone offer a useful protocol or method for cleaning up the DNA?  I'd
really appreciate it.


Mike Sklar

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