dhfr activity assay?
shanth at students.uiuc.edu
Thu Aug 1 15:45:00 EST 1996
I am trying to overproduce some mutants in mammalian cells which are
deficient in their normal activity. The selectable marker that i am using is the dhfr gene
and i am expressing these mutants in CHO/dhfr- cells. I am wondering if
there is an assay for dhfr (dihydrofolate reductase) that will enable me
to select for colonies expressing high levels of the mutant protein. Any
reference or some info would be extremely helpful.
Thanks in advance.
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