PKC assay in Hela cells

David Tak Yan Yu dtyyu at ucla.edu
Thu Aug 1 13:13:53 EST 1996


I am planning to carry out assay of Protein Kinase C acticvity in Hela cells.
I would appreciate if I can have advice about:
1.  Is it necessary to purify the PKC from the membrane fraction by ion 
exchange chromatography prior to assay.  If so, is there a mini method.
2.  Can I use the non-isotopic methods from Pierce or MBL?
Best wishes,
David Yu
dtyyu at ucla.edu



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