SDS PAGE band blurring

[brett]

>My lab has had problems with SDS PAGE band blurring.  A visible line
>demarcates the difference between normal bands on top, and uninterpretable
>blurs below.  Both bands and blurs occur above the dye front.  The line
>has been observed at different positions.  We have also noticed that in
>these gels, the dye front begins to turn from blue to yellow (using
>Bromophenol Blue as a marker).  Anybody know what's going on?  Any help
>would be greatly appreciated. 
> 
>Frances Chen
>fchen at jeeves.ucsd.edu

Sounds as if your lower buffer has little buffering capacity. First thing I'd
do is remake it.


Brett Lindenbach
    
Program in Immunology                              
Washington University - St Louis                  
brett at borcim.wustl.edu                             

"I own my own pet virus. I get to pet and name her." - Cobain