X-gal staining without non-specific in the rat brain ?

Sukwon Lee bioman at plaza.snu.ac.kr
Thu Aug 1 22:22:51 EST 1996


I'm studying in vivo gene transfer using viral vector in the rat brain.

Endogenous beta-galactosidase activity was so effective in the 
hippocampal region that I couldn't detect transgene effect.  :(

I'm trying some breakthrough in different conditions of assay buffer. 
Could anyone mail me some info for me ?

_____________________________________________________

Development and Neuroendocrinology
Dept. of Molecular Biology
Natural Sciences, SNU
Seoul Korea

forfree at plaza.snu.ac.kr





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