Oligonucleotide purification

Lynn Hughes lynn.hughes at jcu.edu.au
Fri Aug 2 13:22:08 EST 1996


Hello, I'm hoping someone can help me with the following problem.  I have 
2 10mer oligonucleotides which I want to anneal, they will give me a 
HindIII sticky end, 6 base pair double stranded region then an EcoRI 
sticky end.  This should be easier enough to do.  However I then need to 
purify this configuration away from my starting  2 single stranded 
oligos, and I am not sure how to do this.  Being such a small fragment of 
DNA I don't think I will be able to use methods such as kits available 
for purifying DNA from agarose gels.  If anyone has any methods I could 
try I'd be very grateful to hear from them

Thanks

Lynn Hughes
Microbiology and Immunology
James Cook University
Townsville, Australia
email lynn.hughes at jcu.edu.au



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