Oligonucleotide purification
Lynn Hughes
lynn.hughes at jcu.edu.au
Fri Aug 2 13:21:12 EST 1996
Hello, I'm hoping someone can help me with the following problem. I have
2 10mer oligonucleotides which I want to anneal, they will give me a
HindIII sticky end, 6 base pair double stranded region then an EcoRI
sticky end. This should be easier enough to do. However I then need to
purify this configuration away from my starting 2 single stranded
oligos, and I am not sure how to do this. Being such a small fragment of
DNA I don't think I will be able to use methods such as kits available
for purifying DNA from agarose gels. If anyone has any methods I could
try I'd be very grateful to hear from them
Thanks
Lynn Hughes
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