SDS PAGE bluring

[Denni Schnapp]

The change of bromophenol blue to yellow is a sure indicator of acidification.
Are you running a discontinuous gel/buffer system? Check that all the buffers
are of appropriate pH. I would like to know what the problem was as it would
be instructive.

Good luck,


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Denni Schnapp		    
Gatty Marine Laboratory    			Fax: (0)1334-463443
University of St. Andrews			e-mail: ds4 at st-and.ac.uk
St. Andrews Fife KY16 8LB   			
Scotland		   	http://www.st-and.ac.uk/~www_sa/personal/ds4/


       		    (Opinions expressed here are my own)

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