Dig-riboprobes and RNase A

Gabriele Kerber kerber at alf.biochem.mpg.de
Thu Aug 1 03:35:49 EST 1996


Kerstin Otte wrote:
> 
> In the Boehringer manual on ribonuclease protection assay it is mentioned
> that digoxigenin may destabilize rna-rna hybrides in such a way that these
> are suceptible to RNase A. Does anyone have any experience with this? How
> does it posthybridization RNAse treatments in in situ hybridizations. BM
> insitu manual says that RNAse A can be used!
> 
> Kerstin Otte
> 
>       /
> |    /
> |   /        Kerstin Otte
> |  /\        Institute for Pathology
> | /  \       Box 7028, SLU              _________________________
> |/    \      75007 Uppsala             /  Tel: 46-18-671199 or
>        \     Sweden                   /   Fax: 46-18-673532
>         \____________________________/    Kerstin.Otte at pat.slu.se





I´m making insituhybridization with Dig-labeled Riboprobes, with a RNAse A treatment afterwards. 
There where never problems with destabilization of the hybrid because of the Dig-molcecules. 
I hope, this will help you!

Gabriele



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