Dig-riboprobes and RNase A
kerber at alf.biochem.mpg.de
Thu Aug 1 03:19:30 EST 1996
Kerstin Otte wrote:
> In the Boehringer manual on ribonuclease protection assay it is mentioned
> that digoxigenin may destabilize rna-rna hybrides in such a way that these
> are suceptible to RNase A. Does anyone have any experience with this? How
> does it posthybridization RNAse treatments in in situ hybridizations. BM
> insitu manual says that RNAse A can be used!
> Kerstin Otte
> | /
> | / Kerstin Otte
> | /\ Institute for Pathology
> | / \ Box 7028, SLU _________________________
> |/ \ 75007 Uppsala / Tel: 46-18-671199 or
> \ Sweden / Fax: 46-18-673532
> \____________________________/ Kerstin.Otte at pat.slu.se
I´m making insituhybridization with Dig-labeled Riboprobes, with a RNAse A treatment afterwards.
There where never problems with destabilization of the hybrid because of the Dig-molcecules.
I hope, this will help you!
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