UV crosslinking of DNA to proteins
sbe at biochem.usyd.edu.au
Fri Aug 2 11:35:04 EST 1996
I am planning a series of experiments in which I want to try and
uv-crosslink a putative DNA-binding protein to both ds and ss human DNA.
I have access to a BioRad GS Gene Linker but the instruction manual
doesn't give any information on this. Does anyone know:
(1) Can I use the Gene Linker for this?
(2) If so, what settings etc should I try?
(3) How should I present the DNA:protein mixtures (in tubes, as drops
on Parafilm, something else?
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