UV crosslinking of DNA to proteins

Simon Easterbrook-Smith sbe at biochem.usyd.edu.au
Fri Aug 2 11:35:04 EST 1996


I am planning a series of experiments in which I want to try and 
uv-crosslink a putative DNA-binding protein to both ds and ss human DNA. 

I have access to a BioRad GS Gene Linker but the instruction manual 
doesn't give any information on this. Does anyone know:

(1)  Can I use the Gene Linker for this?

(2)  If so, what settings etc should I try?

(3)  How should I present the DNA:protein mixtures (in tubes, as drops 
on Parafilm, something else?

Thanks

Simon



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