geneclean: vortexing

Manuel G. CLAROS claros at obelix.cica.es
Fri Aug 2 05:58:24 EST 1996


Desde un rincon del alma, Trevor Bezdek nos confiesa:
 >Any negative ramifications other than for large plasmids?

GeneClean should not be used with large plasmids. Bio101 says that 15 kb
is the limit, but I have found that 10 kb plasmids smear after this
treatment.

I have moved to the freeeze-squize long time ago: better yields than
GeneClean and no limit in size.

Dr Dienei :-) 
-- 
Manuel G. CLAROS     -. .-.   .-. .-.   .       -> claros at cica.es <-
Biologia Molecular   ||X|||\ /|||X|||\ /|   No quiero cambiar, no puedo,
Facultad Ciencias    |/ \|||X|||/ \|||X||   no quiero ser una frase mas.
E-29071 Malaga       '   `-' `-'   `-' `-         (Esclarecidos)



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