Removal of Ethidium Bromide

MELANSOND melansond at am.abru.cg.com
Fri Aug 2 15:12:41 EST 1996


Edward,	
	No need for organics...

Make a 1 to 1 solution of 5 M NaCl with 100 % Isopropanol.  It will precipitate
white stuff in the bottom, and form two layers.  Use the top layer to extract
the EtBr from your samples (e.g. Add equal volume to your sample and mix. Allow
layers to form, Discard top layer, and repeat until no pink is visible in top
layer, and then once more. 
	

Then Dilute the bottom layer in 2 volumes of water, and EtOH precipate as
normal.

Good Luck
D




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