freeze-squeeze (Re: geneclean: vortexing)
trevor at leland.stanford.edu
Fri Aug 2 23:22:53 EST 1996
In article <4tsn0g$npa at erika.cica.es>, claros at obelix.cica.es (Manuel G.
> I have moved to the freeeze-squize long time ago: better yields than
> GeneClean and no limit in size.
The problem with freeze-squeeze is the huge elution volume. When you use
any reasonable amount for a ligation reaction the yields are much lower
than for GeneClean. Also, you have to use miniscule amounts to avoid
inhibiting enzymatic reactions. Are my limited experiences with
freeze-squeeze, which is by far the simplest cleanup method possible
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