GST-fusions and proteolysis

BIOTEC54 biotec54 at
Sat Aug 3 10:08:35 EST 1996

I've been working with several GST-fusion constructs that were built using
the pGEX-2T vector. While I usually see good expression of my fusion
protein....upon purification via GSH affinity...I also seem to co-purify
material corresponding to the GST portion only. I'm wondering if the
inclusion of the thrombin cleavage site at the fusion junction is being
cleaved via a host protease? Has anyone removed the thrombin site and seen
only full-length protein?

More information about the Methods mailing list