trehalose determination

Sanford Silverman silvermans at pt.cyanamid.com
Mon Aug 5 14:40:49 EST 1996


Ed Bellion wrote:
> 
> Does anyone know of a good method for trehalose
> determination in cell extracts using either HPLC or GC.
> The well-known colorimetric anthrone  method is
> unsuitable for our needs as it gives poor results
> with smaller concentrations.
> 
> Thanks
> 
> Ed Bellion

You can get commercially available trehalase (from Sigma, I think) and 
then measure glucose.

-------Sandy
-- 
Sanford Silverman               >Opinions expressed here are my own< 
American Cyanamid  
silvermans at pt.cyanamid.com                      "Yeast is Best"



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