trehalose determination

Sanford Silverman silvermans at
Mon Aug 5 14:40:49 EST 1996

Ed Bellion wrote:
> Does anyone know of a good method for trehalose
> determination in cell extracts using either HPLC or GC.
> The well-known colorimetric anthrone  method is
> unsuitable for our needs as it gives poor results
> with smaller concentrations.
> Thanks
> Ed Bellion

You can get commercially available trehalase (from Sigma, I think) and 
then measure glucose.

Sanford Silverman               >Opinions expressed here are my own< 
American Cyanamid  
silvermans at                      "Yeast is Best"

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