SDS PAGE band blurring

Achim Recktenwald achim at ibex.ca
Mon Aug 5 13:47:04 EST 1996


brett wrote:
> 
[snip]

> > We have also noticed that in
> >these gels, the dye front begins to turn from blue to yellow (using
> >Bromophenol Blue as a marker). 
> >
> >Frances Chen
> >fchen at jeeves.ucsd.edu
> 
> Sounds as if your lower buffer has little buffering capacity. First thing I'd
> do is remake it.
> 

Bromphenolblue turns yellow, when the pH is too low; redo your buffer.



Achim



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