HELP HELP HELP needed with RNA extraction
alex emil kentsis
aekentsi at woodlawn.uchicago.edu
Mon Aug 5 21:31:28 EST 1996
i have never had trouble with guanidine-based extractions. gibco has
a reagent mexture called trizol engineered for efficient rna extractions.
you may want to look into it.
Paul A Bucciaglia (bucci001 at maroon.tc.umn.edu) wrote:
: I don't have any experienc with guanidine-based RNA extractions, but I
: have had pretty good luck with either the standard phenol:chloroform
: based extraction or a modified protocol by Schultz et al (Plant Mol.
: Biol Reporter 12 (4), p310. 1994). This worked very well when extracting
: RNA from Populus leaves, although I didn't try any woody tissues. Doesn't
: require a CsCl step, which I personally hate doing. RNA was clean,
: intact, and reverse transcribed well.
: Good luck,
: Paul Bucciaglia
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