Reasons for PCR failure

Susan B. McLeskey smcleske at acpub.duke.edu
Mon Aug 5 19:24:12 EST 1996


With regard to wacky PCR suggestions:

One time I was using pfu for PCR and getting the 
lowest yields imaginable. I could barely convince 
myself that a band was there if I loaded the whole
reaction in one lane.  If I lowered the annealing
temperature, I got crap;  If I raised it, I got nothing.
Then, I read the pfu package insert that suggested
using 10 % glycerol or 10 % DMSO. 

Both worked like gangbusters!!

Susan McLeskey



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