UV crosslinking of DNA to proteins
Luc.Vallieres at crchul.ulaval.ca
Mon Aug 5 17:12:48 EST 1996
> I am planning a series of experiments in which I want to try and
> uv-crosslink a putative DNA-binding protein to both ds and ss human DNA.
> I have access to a BioRad GS Gene Linker but the instruction manual
> doesn't give any information on this. Does anyone know:
> (1) Can I use the Gene Linker for this?
> (2) If so, what settings etc should I try?
> (3) How should I present the DNA:protein mixtures (in tubes, as drops
> on Parafilm, something else?
I successfully used this method in a previous study. See the following paper:
Roy, R., L. Vallieres, S. Leclerc and S. Guerin. 1994. The rat growth hormone
proximal silencer contains a novel DNA-binding site for multiple nuclear
that represses basal promoter activity. Eur. J. Biochem. 225: 419-432.
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