HELP HELP HELP needed with RNA extraction
Paul A Bucciaglia
bucci001 at maroon.tc.umn.edu
Mon Aug 5 16:47:19 EST 1996
I don't have any experienc with guanidine-based RNA extractions, but I
have had pretty good luck with either the standard phenol:chloroform
based extraction or a modified protocol by Schultz et al (Plant Mol.
Biol Reporter 12 (4), p310. 1994). This worked very well when extracting
RNA from Populus leaves, although I didn't try any woody tissues. Doesn't
require a CsCl step, which I personally hate doing. RNA was clean,
intact, and reverse transcribed well.
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