long PCR primer

Chen Ho An chen at bsm.bioc.ucl.ac.uk
Mon Aug 5 13:52:40 EST 1996

A member of my group did a PCR using a very long oligo ( >80 bases) as
the 3' primer.  However after the PCR product is TA-cloned into pGEM and
sequenced, chunks of the 3' end (~40 bases) seems to have disappear.  I
wonder if this is a general problem of PCR  using long primer or a
problem with the synthesis of the primer? Or may be there is a problem
with the pGEM?  Any suggestion as to what is happening? Also how do you
check that you primer is OK?


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