about transformation of DNA into E.Coli
edgar at cifn.unam.mx
Tue Aug 6 16:40:11 EST 1996
We had always obtained much better results with electroporation.
Nevertheles, we havent used plasmids larger than 15 kb, i dont know if
the efficency could be reduced if you use larger plasmids or cosmids
I hope this helps
Edgar Valencia Morales
Departamento de Genetica Molecular
CIFN - UNAM
e-mail edgar at cifn.unam.mx
On 6 Aug 1996, Jin-seon Im wrote:
> hello fellas..
> does anyone try to compare transforming methods - electroporation and
> heat shock ?
> which one is better?
> Thanks in advance
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