about transformation of DNA into E.Coli

Edgar Valencia edgar at cifn.unam.mx
Tue Aug 6 16:40:11 EST 1996


Hi Jin-seon

We had always obtained much better results with electroporation. 
Nevertheles, we havent used plasmids larger than 15 kb, i dont know if 
the efficency could be reduced if you use larger plasmids or cosmids
I hope this helps
                          Edgar Valencia Morales
                    Departamento de Genetica Molecular
                               CIFN - UNAM
                                  MEXICO
                        e-mail edgar at cifn.unam.mx


On 6 Aug 1996, Jin-seon Im wrote:

> 
> 
> hello fellas..
> 
> does anyone try to compare transforming methods - electroporation and 
> heat shock ?
> which one is better?
> 
> 
> Thanks in advance
> 
> jsim
> 
> 
> 
> 



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