Help with PCR library screening

John Brennand john.brennand at gbapr.zeneca.com
Tue Aug 6 04:09:35 EST 1996


Miguel

Yep we have done this several times although i'm not sure whether we are 
following the protocol you cite.

We plate out 1 million plaques or colonies at 50,000/plate, make lysates 
and PCR the lysate.  Positive aliquots are replated at 10,000 plate and 
rescreened.  We repeat this cycle until we have positive aliquots of 
<1000 clones then screen by hybridisation.

It works and it is easier than it sounds.

john





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