Help with PCR library screening

[John Brennand]

Miguel

Yep we have done this several times although i'm not sure whether we are 
following the protocol you cite.

We plate out 1 million plaques or colonies at 50,000/plate, make lysates 
and PCR the lysate.  Positive aliquots are replated at 10,000 plate and 
rescreened.  We repeat this cycle until we have positive aliquots of 
<1000 clones then screen by hybridisation.

It works and it is easier than it sounds.

john