Help with PCR library screening
john.brennand at gbapr.zeneca.com
Tue Aug 6 04:09:35 EST 1996
Yep we have done this several times although i'm not sure whether we are
following the protocol you cite.
We plate out 1 million plaques or colonies at 50,000/plate, make lysates
and PCR the lysate. Positive aliquots are replated at 10,000 plate and
rescreened. We repeat this cycle until we have positive aliquots of
<1000 clones then screen by hybridisation.
It works and it is easier than it sounds.
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