Your basic stupid question about PCR cloning

Chromosome Terror abrdlher at reading.ac.uk
Tue Aug 6 14:52:34 EST 1996


On Fri, 2 Aug 1996, Frederick Garbrecht wrote:

> I have a 23 kB fragment that I PCRd and would like 
> to put in a cloning vector.  Not knowing much about 
> cloning vectors, can I assume that a fragment of 
> that size would not go happily into your standard 
> plasmid?  Should I put it into lambda?

I think so. For 20Kb inserts I always used cosmids OK.

McNach




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