Your basic stupid question about PCR cloning
abrdlher at reading.ac.uk
Tue Aug 6 14:52:34 EST 1996
On Fri, 2 Aug 1996, Frederick Garbrecht wrote:
> I have a 23 kB fragment that I PCRd and would like
> to put in a cloning vector. Not knowing much about
> cloning vectors, can I assume that a fragment of
> that size would not go happily into your standard
> plasmid? Should I put it into lambda?
I think so. For 20Kb inserts I always used cosmids OK.
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