MutS gel mobility shift

Les Hoffman lhoffman at midplains.net
Tue Aug 6 17:28:56 EST 1996


Has anyone found a reliable protocol for gel mobility shift assays using 
MutS protein?  Heteroduplexes and homoduplexes of PCR products are both 
being apparently bound by MutS protein because the gel shift is similar 
for both. Inclusion of polymers such as poly(dA-dT) does not prevent the 
mobility shifting of the homoduplexes.  Any ideas?

			Thanks,
			Les Hoffman





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