lysis of RAW macrophage-like cell line

Jim Graham graham at biodec.wustl.edu
Tue Aug 6 12:05:45 EST 1996


>Anyone know any great, relatively gentle methods of lysing
>RAW cells?

I've also had some problems lysing cultured human primary monocytes
with 0.1% SDS. At least there was no gross "clearing" of the plastic
surface as I expected. (Anyone know if this residual material is 
an exrtracellular matrix of some kind?)

Other cells lines of interest here might be THP-1 and the MonoMac line.
Any other good choices for human macorphage-like cell lines?

Any correspondence on harvesting bacteria infecting macrophage 
or monocytic cell lines most appreciated.

Regards,

Jim
J. Graham PhD 
Biology Department 
Washington University of St. Louis 



More information about the Methods mailing list