lysis of RAW macrophage-like cell line

[Jim Graham]

>Anyone know any great, relatively gentle methods of lysing
>RAW cells?

I've also had some problems lysing cultured human primary monocytes
with 0.1% SDS. At least there was no gross "clearing" of the plastic
surface as I expected. (Anyone know if this residual material is 
an exrtracellular matrix of some kind?)

Other cells lines of interest here might be THP-1 and the MonoMac line.
Any other good choices for human macorphage-like cell lines?

Any correspondence on harvesting bacteria infecting macrophage 
or monocytic cell lines most appreciated.

Regards,

Jim
J. Graham PhD 
Biology Department 
Washington University of St. Louis