RNA Quantification...

[Colin Dolphin]

Subject: Re: RNA Quantification...
From: Allan Colthart, ac50 at le.ac.uk
Date: Tue, 06 Aug 1996 16:55:41 -0700
In article <3207DB7D.597F at le.ac.uk> Allan Colthart, ac50 at le.ac.uk
writes:
>Edouard Lauzier wrote:
>> 
>> If  I re-quantify RNA samples ( five years old... ) and the values I
get
>> give me higher 260/280 ratio but less RNA...
>> 
>> Is there any logical explanation ??
>> 
>> Thanks...
>> --
>> Edouard Lauzier, B.Sc.                           elauzier at fse.ulaval.ca
>> Physical Activity Science Laboratory       (418) 656-2131 #2929
>> (Laval University)  G1K 7P4
>> CANADA
>
>Someone please correct me if I'm wrong on this, but 5 year old RNA 
>samples are pretty unlikely to contain much in the way of useable RNA.

I routinely use RNA stored >5 yrs - if RNA is isolated and stored
correctly then it should be pretty stable. RNA degrades because of
RNase activty, remove this and RNA should last for yonks at -80.

Colin