c.t.dolphin at qmw.ac.uk
Wed Aug 7 03:03:21 EST 1996
Subject: Re: RNA Quantification...
From: Allan Colthart, ac50 at le.ac.uk
Date: Tue, 06 Aug 1996 16:55:41 -0700
In article <3207DB7D.597F at le.ac.uk> Allan Colthart, ac50 at le.ac.uk
>Edouard Lauzier wrote:
>> If I re-quantify RNA samples ( five years old... ) and the values I
>> give me higher 260/280 ratio but less RNA...
>> Is there any logical explanation ??
>> Edouard Lauzier, B.Sc. elauzier at fse.ulaval.ca
>> Physical Activity Science Laboratory (418) 656-2131 #2929
>> (Laval University) G1K 7P4
>Someone please correct me if I'm wrong on this, but 5 year old RNA
>samples are pretty unlikely to contain much in the way of useable RNA.
I routinely use RNA stored >5 yrs - if RNA is isolated and stored
correctly then it should be pretty stable. RNA degrades because of
RNase activty, remove this and RNA should last for yonks at -80.
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