Reasons for PCR failure....?

:-Peter obrien at pharm.med.upenn.edu
Wed Aug 7 14:07:51 EST 1996


I always play my favorite Grateful Dead song (Crazy Fingers) while I prep
the hot start.  I don't know if it helps the reaction, but I always smile,
smile, smile!

I also never use autoclaved water, rather I filter sterilize Milli-Q
purified water.  Apparently some autoclaves can leave residual solutes.

-- 
:-Peter





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