about transformation of DNA into E.Coli

Paul N Hengen pnh at ncifcrf.gov
Wed Aug 7 13:15:07 EST 1996


Jeremy Marcus (marcusj at helix.mgh.harvard.edu) wrote:

> Sailesh.Surapureddi at mcb.liu.se (Sailesh Surapureddi) wrote:
> 
> : The answer for your question is NONE. 3 min on ice is much better
> : then electroporation or heat shock.
> : 
> : Sailesh.
> 
> WHAT???!!!!

Yeah, where does this come from? From all of everything I've come across...

electroporation > chemical transformation + heat shock > chemical transformation

@article{Hengen1995Juntibs,
author = "P. N. Hengen",
title = "Methods and reagents - Electro-transformation of
{{\em Escherichia coli}} with plasmid {DNA}",
journal = "Trends in Biochemical Sciences",
volume = "20",
number = "6",
pages = "248-249",
month = "jun",
year = "1995"}

@article{Hengen1996Febtibs,
author = "P. N. Hengen",
title = "Methods and reagents - Preparing ultra--competent
{{\em Escherichia coli}}",
journal = "Trends in Biochemical Sciences",
volume = "21",
number = "2",
pages = "75-76",
month = "feb",
year = "1996"}

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