Help: genomic DNA purification

Hongyu yang yang at tifton.cpes.peachnet.edu
Wed Aug 7 20:26:10 EST 1996


Hi netters:

We were able to isolate genomic DNA using 2xCTAB protocol from plant leaves. Following 
isopropanol addition there was a big cloud of stuff (we thought it was the DNA 
precipitation). We dried the "DNA pellet" and tried to redisolve it in TE, some gelly 
stuff could not be disolved even with 65C water bath. 

I'll appreciate any advices to remove the "gelly stuff" from my DNA solution.


Thanks


HY



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