RAPD Trouble!!!

Hongyu yang yang at tifton.cpes.peachnet.edu
Wed Aug 7 20:09:06 EST 1996

Roland Koelliker wrote:
> Dear netters
> I'm using RAPD to detect genetic variability in festuca pratensis (meadow
> fescue). All worked fine so far, after initial problems I found a set of 20
> primers that produce nice polymorphic bands. All of a sudden these primers
> stop to work. I tried everything (new polymerase, new buffer, mgcl, dntp,
> and so on) but still I do not get the same nice results as before. Since it
> once worked and I already have some nice pictures I don't want to play
> around with the reaction conditions again. Does anybody have an idea what's
> going on here? I'm quite desperate.
> Thank your help
> Roland
> Roland Koelliker
> Swiss Fed Inst Technol
> Plant Sciences
> ETH-Zentrum, LFW-C47
> CH-8092 Zurich
> Tel ++41-1-632 4890
> Fax ++41-1-632 1153
> e-mail: koelliker at ipw.agrl.ethz.ch

Is your DNA ok?


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