graham at biodec.wustl.edu
Wed Aug 7 19:05:28 EST 1996
Here is what the hit or miss sloppy database at Biotech Net
(http://www.biotechniques.com) gives. I hear that the "Journal"
Biotechniques is not avialable in the UK
Title: Single-Step Purification of a Thermostable DNA Polymerase
Expressed in Escherichia coli
Desai, U.J. ; Pfaffle, P.K.
Single-Step Purification of a Thermostable DNA Polymerase
Expressed in Escherichia coli. The coding region of the gene for Taq DNA
polymerase has been cloned into the common vector pUC18. Using a
single-step procedure, large amounts of active enzyme can be purified from
Escherichia coli carrying this construct. This procedure takes
advantage of the thermostable properties of the DNA polymerase. This simple
procedure gives very high yields of essentially homogenous, highly
active enzyme suitable for use in molecular biological applications.
Yields are over two orders of magnitude greater than available with current
(Biotechniques, Short Technical Report 19:780-784)
More information about the Methods