5 min transformation E. coli

KHUANG at CHEMVX.TAMU.EDU KHUANG at CHEMVX.TAMU.EDU
Wed Aug 7 17:58:42 EST 1996


What I want to say is electroporation can give more higher transformation
efficiency than the 5 min protocol if you use the same amount of DNA.
I only could get 106-107 transformants/ug DNA by using 5 min transformation
protocol, but I can get 109-1010 transformants by electroporation. I want to 
know how you make competent cell.
Huang



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