5 min transformation E. coli

[Sailesh Surapureddi]

>Although Dr. Sailesh said 5 min transformation E. coli is more better than
>electroporation. I am not sure it is true. It depends how much DNA  you used.
>I tried this 5 min transformation protcol for a while, but now I came back to
>use electroporation.
>
>Huang


Its not the quantity but the quality of the DNA is imp for higher
transformations. I use quiagen purified DNA and I get very good results.

Sailesh.

Dr.Sailesh Surapureddi
Plan 12; Inst. for Cellbiologi
Halsouniversitetet
S 581 85, Linkoping
Sweden.

Tel: +46-13-223917 (W) +46-13-138839 (R)
Fax: +46-13-224149
E.mail: Saisumcb at hulio.liu.se
        Saisu at mcb.liu.se