5 min transformation E. coli
Sailesh.Surapureddi at mcb.liu.se
Wed Aug 7 17:45:28 EST 1996
>Although Dr. Sailesh said 5 min transformation E. coli is more better than
>electroporation. I am not sure it is true. It depends how much DNA you used.
>I tried this 5 min transformation protcol for a while, but now I came back to
Its not the quantity but the quality of the DNA is imp for higher
transformations. I use quiagen purified DNA and I get very good results.
Plan 12; Inst. for Cellbiologi
S 581 85, Linkoping
Tel: +46-13-223917 (W) +46-13-138839 (R)
E.mail: Saisumcb at hulio.liu.se
Saisu at mcb.liu.se
More information about the Methods