5 min transformation E. coli

Sailesh Surapureddi Sailesh.Surapureddi at mcb.liu.se
Wed Aug 7 17:45:28 EST 1996

>Although Dr. Sailesh said 5 min transformation E. coli is more better than
>electroporation. I am not sure it is true. It depends how much DNA  you used.
>I tried this 5 min transformation protcol for a while, but now I came back to
>use electroporation.

Its not the quantity but the quality of the DNA is imp for higher
transformations. I use quiagen purified DNA and I get very good results.


Dr.Sailesh Surapureddi
Plan 12; Inst. for Cellbiologi
S 581 85, Linkoping

Tel: +46-13-223917 (W) +46-13-138839 (R)
Fax: +46-13-224149
E.mail: Saisumcb at hulio.liu.se
        Saisu at mcb.liu.se

More information about the Methods mailing list