about transformation of DNA into E.Coli

H. ROYCHOWDHURY hroychow at NMSU.EDU
Wed Aug 7 17:42:06 EST 1996


Somebody mentioned something about transformation of bacteria (with 
plasmids) without having to resort to shocking the cells at 37-42 C, and 
simply by leaving the cells on ice for 3 min. I have not come across any 
such method so far. Has anybody tried this? Is it an established method? 
How does it work? Does it mean that the 15-20 min on ice followed by HS 
can simply be cut down to just 3 min on ice? I would like some reference to 
this protocol.

			>>>>>>>>>>>>>>>>>>>>>>>>>>>
			  Hiranya S. Roychowdhury, Ph.D.
   			  Plant Genetic Engineering Lab.
			  Box 3GL, NM State Univ.
			  Las Cruces, NM 88003
			  Phone: (505) 646-5785
			  hroychow at nmsu.edu
			<<<<<<<<<<<<<<<<<<<<<<<<<<<




More information about the Methods mailing list