Hi Dr. SaileshIce transformation question

[Edgar Valencia]

How do you prepare your competent cell, calcium method, Inoue method 
which one?
Do you have a reference for this ice transformation method?
Thanks

                          Edgar Valencia Morales
                    Departamento de Genetica Molecular
                               CIFN - UNAM
                                  MEXICO
                        e-mail edgar at cifn.unam.mx