Your basic stupid question about PCR cloning

Vlad Svetlov vvsvetlov at utmem1.utmem.edu
Tue Aug 6 19:14:32 EST 1996


In article <32025974.9F9 at lac.jci.tju.edu>, Frederick Garbrecht
<fgarbrec at lac.jci.tju.edu> wrote:

> I have a 23 kB fragment that I PCRd 
>  Should I put it into lambda?


Depends on what you gonna do with that clone of yours later. There are
several options of cloning large fragments of DNA incl. phages, cosmids
and YACs. They offer some choices in regard to propagation, manipulation
and utilization of your construct, so decide what you want out of your
clone first. 
Regards,
Vlad



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