Double stranded dna Sequencing help

Shiao Wang swang at
Wed Aug 7 12:48:07 EST 1996

I am having some problems with the sequencing due to too
> > much stacking of the bands. I know this can be caused because of
> > secondary structures and so on, but I would like to know if somebody can
> > pass me on, let's say, the latest version of the best method for
> > sequencing doubled stranded DNA. I use the Pharmacia kit, does anyone
> > uses something else besides what they provide ?

We have used cycle sequencing with success. The higher temperature helps.
Shiao Y. Wang
Univ. of Southern Mississippi
sywang at

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